家兔股静脉血栓和股动脉血栓的定位诊断

为研究99m锝标记的D 二聚体 (D dimer,DD)单抗在静脉血栓和动脉血栓靶向定位诊断中的应用 ,采用外科手术的方法复制家兔股静脉和股动脉血栓模型 ,将99m锝标记的抗DD单抗注入动物血管内 ,以放射免疫显像检测技术观察放射性核素在血流和血栓部位的分布。结果 :血栓形成术后 2d ,血栓处放射性99m锝的分布较健侧和正常兔明显增多 ,而单纯钳夹血管未能引起局部放射性物质的堆积。注射99m锝标记的单抗后 ,动物无明显不良反应 ,主要器官无明显病理学改变。研究结果提示 :99m锝标记抗DD单抗可对实验性股静脉血栓和股动脉血栓作出快速、准确的定位诊断

Localization for Rabbit Femoral Venous and Arterious Thrombus

The aim was to evaluate the precious localization of femoral venous and arterious thrombus targeted by 99mTc monoclonal antibody. Antibody labeled with 99mTc-anti-D-dimer was administrated into femoral vein and artery of the rabbits that were subjected to the thrombotic operation. Using radioimmunoimaging technique, the distribution, blood flow and radio-specificity of 99mTc-anti-D-dimer within thrombus were analyzed both in intact and thrombolized animals. Results: 99mTc-anti-D-dimer was specifically concentrated in thrombus, not in clamp and normal side. In addition, no abnormal reaction resulted from the injection of 99mTc-anti-D-dimer and no pathologic changes in main organs were found. Conclusion: the 99mTc-labeled anti-D-dimer antibody will be one of potentially tools for experimental thrombus diagnosis.