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| 骨髓增生异常综合征中基质细胞衍生因子1与血管新生及细胞凋亡的相关性研究 | |
| 作者姓名： | Zhi YQ Zhang YZ Zhao HF Zhao DD Xia B Wu XX Da WM |
| 作者单位： | 1. 天津医科大学附属肿瘤医院血液科,天津,300060 2. 天津医科大学附属肿瘤医院血液科,天津300060;解放军总医院血液科,北京100853 3. 解放军总医院第一附属医院血液科,北京,100037 4. 解放军总医院血液科,北京,100853 |
| 基金项目： | 国家自然基金面上项目(编号30672208) |
| 摘 要： | 本研究探讨骨髓增生异常综合征(MDS)中骨髓基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)的表达,及其与骨髓CD34+细胞的凋亡和骨髓血管新生的关系。搜集40例MDS病例,根据IPSS积分分为低危和高危2组,采集患者的骨髓穿刺物和活检标本。检测骨髓血浆中SDF-1的含量,骨髓CD34+细胞的凋亡率,骨髓活检标本的微血管密度(MVD)。结果显示:SDF-1α在低危组和高危组的表达量分别为(2313±417)pg/ml,(1241±501)pg/ml,前者显著高于后者(p<0.05);且2组的表达率均显著高于健康正常者(710±153)pg/ml(p<0.05);Annexin-Ⅴ/PI双染色显示,低危MDS患者CD34+细胞的凋亡率为(54.75±14.15)%,显著高于对照组的(18.51±8.66)%和高危组的(23.96±12.20)%(p<0.05),后两者相比差异无显著性(p>0.05);相关分析显示:低危组中骨髓SDF-1含量与CD34+细胞凋亡率呈正相关(p<0.05);在高危组中骨髓SDF-1含量与MVD呈现正相关(p<0.05)。结论:骨髓SDF-1含量、CD34+细胞凋亡和MVD在MDS中存在显著异常,在不同风险度的病例中也有明显的不同,且SDF-1水平与骨髓细胞的凋亡和血管新生具有相关性。 |
| 关 键 词： | 骨髓增生异常综合征 基质细胞衍生因子-1 细胞凋亡 微血管密度 |
Correlation of stromal cell derived factor-1 with angiogenesis and cell apoptosis in myelodysplastic syndromes |
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| Zhi YQ,Zhang YZ,Zhao HF,Zhao DD,Xia B,Wu XX,Da WM.Correlation of stromal cell derived factor-1 with angiogenesis and cell apoptosis in myelodysplastic syndromes[J].Journal of Experimental Hematology,2011,19(6):1438-1442. | |
| Authors: | Zhi Ya-Qin Zhang Yi-Zhuo Zhao Hai-Feng Zhao Dan-Dan Xia Bing Wu Xiao-Xiong Da Wan-Ming |
| Institution: | ZHI Ya-Qin1,ZHANG Yi-Zhuo1,2,ZHAO Hai-Feng1,ZHAO Dan-Dan3,XIA Bing1,WU Xiao-Xiong3,DA Wan-Ming2 1Department of Hematology,Tianjin Medical University Cancer Hospital,Tianjin 300060,China,2Department of Hematology,Chinese PLA General Hospital,Beijing 100853,3Department of Hematology,the First Affiliated Clinical Hospital of Chinese PLA General Hospital,Beijing 100037 |
| Abstract: | The study was aimed to investigate the expression of stromal cell derived factor(SDF-1) in bone marrow(BM) and its relation with apoptosis of BM CD34+ cell and angiogenesis in myelodysplastic syndrome(MDS).40 patients with MDS were divided into low-risk group and high-risk group according to IPSS score system.BM samples were collected.SDF-1 levels,the apoptosis of CD34+ cells and microvessel density(MVD) of BM were detected by ELISA,flow cytometry and immunochemistry,respectively.The results showed that the... |
| Keywords: | myelodysplastic syndrome stromal cell derived factor-1 apoptosis microvessel density |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |