| 大鼠骨髓间充质干细胞体外培养与慢病毒转染方法的建立 |
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| 引用本文： | Zhang HS,Fang JP,Su HB,Yang M. 大鼠骨髓间充质干细胞体外培养与慢病毒转染方法的建立[J]. 中国实验血液学杂志, 2011, 19(6): 1472-1476 |
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| 作者姓名： | Zhang HS Fang JP Su HB Yang M |
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| 作者单位： | 1. 中山大学孙逸仙纪念医院儿科,广东广州,510120
2. 复旦大学附属儿科医院消化科,上海,201102 |
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| 基金项目： | 广东省科技计划项目,编号2010B060900027 |
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| 摘 要： | 本研究建立大鼠骨髓间充质干细胞(BMMSC)体外分离培养、表型鉴定和标记的方法,为临床进行细胞移植治疗提供合格的细胞.无菌条件下取大鼠股骨、胫骨,用冲洗法冲出骨髓,贴壁培养法分离纯化BMMSC,体外扩增;流式细胞术进行免疫表型鉴定,并进行成骨,成脂分化鉴定;利用携带绿色荧光蛋白(GFP)基因的慢病毒液转染大鼠BMMSC...
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| 关 键 词： | 骨髓间充质干细胞 慢病毒转染 贴壁培养法 |
Culture in vitro and lentivirus transfection of rat mesenchymal stem cells |
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| Zhang Hong-Shan,Fang Jian-Pei,Su Hao-Bin,Yang Min. Culture in vitro and lentivirus transfection of rat mesenchymal stem cells[J]. Journal of experimental hematology, 2011, 19(6): 1472-1476 |
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| Authors: | Zhang Hong-Shan Fang Jian-Pei Su Hao-Bin Yang Min |
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| Affiliation: | Department of Pediatric, Sun Yat-Sen University, Guangdong Province, China. |
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| Abstract: | This study was purposed to establish the methods for isolation,culture,identification and labeling of bone marrow mesenchymal stem cells(BMMSC),so as to provide quantified seed cells for cell transplantation.Bone marrow was collected from SD rat by flushing femur and tibias under sterile condition and BMMSC were purified by adherent culture and amplified in vitro.The immunophenotypes of BMMSC were identified by flow cytometry,the ability of differentiation to osteogenic and adipogenic lineages was detected ... |
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| Keywords: | bone marrow mesenchymal stem cell lentivirus transfection adherent culture |
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