佐使配伍对含关木通的龙胆泻肝汤全方肾毒性作用的影响

目的观察单味关木通、龙胆泻肝汤和去当归、生地、甘草的龙胆泻肝汤给药后大鼠肾功能和组织形态学变化。方法大鼠随机分为正常对照组、单味关木通组、龙胆泻肝汤组和龙胆泻肝汤去归地草方组,正常对照组给予等量蒸馏水,后3组分别按6、17.5、14.5 g/kg给以相应方药的水煎液灌胃,各组关木通含量均为6 g/kg,连续8周。给药结束后,测定大鼠血、尿生化指标,观察肾脏组织形态学改变。结果各水煎液灌胃组,均有明显肾损伤表现,其中以单味关木通组损伤最为严重,龙胆泻肝汤组较轻,去归地草的龙胆泻肝汤组较龙胆泻肝汤组损伤重,个别指标甚至高于单味关木通组。结论实验剂量的关木通与配伍关木通的两个复方均可引起肾损伤,龙胆泻肝汤去当归、生地、甘草方的肾毒性较龙胆泻肝汤强。提示含关木通配伍的复方的毒性作用不等于关木通,龙胆泻肝汤方中当归、生地、甘草的配伍对关木通的肾损伤有一定的减毒作用。

Influence of compatibility of whole Longdanxiegan Decoction (containing aristolochiae manshuriensis)on nephrotoxicity

Objective To observe the changes of renal function and histomorphology in rats treated with aristolochiae manshuriensis(AM),Longdanxiegan Decoction(LD) and Modified Longdanxiegan Decoction(MLD,removed angelica sinensis,rehmannia dride rhizome and glycyrrhizae uralensis).Method All SD rats were randomly divided into the normal control group(group 1),AM group(group 2),LD group(group 3) and MLD group(group 4).Group 1 was given distilled water orally for 8 weeks,and group 2,3 and 4 were given respectively AM(6 g/kg),LD(17.5 g/kg,containing AM 6 g/kg) and MLD(14.5 g/kg,containing AM 6 g/kg) all for 8 weeks.The biochemical indexes in serum and urine,and renal histomorphological changes in rats were detected at the end of 8th week.Result In group 2,3 and 4 the rats all had significant renal lesion,which was the most serious in group 2,and less in group 3,but in group 4 the renal lesion was serious too and some indexes was even higher than those in group 2.Conclusion AM,LD and MLD all can induce the renal lesion and MLD has stronger nephrotoxicity.It suggests that the toxicity of formulas containing AM is not as the same as that of AM.Angelica sinensis,rehmannia dride rhizome and glycyrrhizae uralensis contained in LD may inhibit the nephrotoxicity of AM.