| 库拉索芦荟多糖的分离纯化和含量测定 |
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| 引用本文： | 陈伟,陈俊,林新华,黄丽英,罗红斌. 库拉索芦荟多糖的分离纯化和含量测定[J]. 中国现代应用药学, 2005, 22(2): 131-133 |
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| 作者姓名： | 陈伟 陈俊 林新华 黄丽英 罗红斌 |
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| 作者单位： | 福建医科大学药学系,福建,福州,350004 |
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| 基金项目： | 福建省科技厅重点攻关课题(2002I015) |
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| 摘 要： | 目的分离纯化库拉索芦荟多糖并建立含量测定方法.方法醇沉法提取库拉索芦荟多糖,Sephadex G-100凝胶柱色谱纯化.以甘露糖为标准,苯酚-硫酸法测定库拉索芦荟多糖含量.结果经分离纯化得到的主要组分库拉索芦荟多糖Ⅰ含量为81.11%,测定平均回收率为100.3%.结论该方法可从库拉索芦荟中提取分离纯度较高的多糖,含量测定方法简便、快速、重现性好.
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| 关 键 词： | 库拉索芦荟 多糖 分离 纯化 测定 |
| 文章编号： | 1007-7693(2005)02-0131-03 |
Isolation,purification and determination of the polysaccharide of Aloe barbadensis |
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| CHEN Wei,CHEN Jun,LIN Xin-hu,HUANG Li-ying,LUO Hong-bin. Isolation,purification and determination of the polysaccharide of Aloe barbadensis[J]. The Chinese Journal of Modern Applied Pharmacy, 2005, 22(2): 131-133 |
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| Authors: | CHEN Wei CHEN Jun LIN Xin-hu HUANG Li-ying LUO Hong-bin |
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| Abstract: | OBJECTIVE To isolate, purify the polysac ch aride of Aloe barbadensis Miller and establish the content determination method.METHOD The polysaccharide was precipitated with ethanol and fur ther purified on Sephadex G-100 column. Its total soluble sugar content was det ected colorimetrically by the phenol-sulfuric acid method with mannose as stand ard. RESULTS The total sugar content of polysaccharide I was 81.11%. The average recovery was 100.3%. CONCLUSION It is possible to extract and isolate purified polys accharide from Aloe barbadensis Miller. And the determination method is simple, rapid and well reproducible. |
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| Keywords: | Aloe barbadensis Miller polysaccharide isolatio n purification determination |
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