The effect of current used bone substitution materials and platelet-rich plasma on periosteal cells by ectopic site implantation: An in-vivo pilot study |
| |
| Authors: | Philipp Metzler Cornelius von Wilmowsky Robert Zimmermann Jörg Wiltfang Karl Andreas Schlegel |
| |
| Institution: | 1. Alps Electric Co., Ltd., Higashitakami 1-3-5, Nagaoka, Niigata 940-8572, Japan;2. Department of Earth and Space Science, Graduate School of Science, Machikaneyama-cho 1-1, Osaka University, Toyonaka, Osaka 560-0043, Japan;1. Department of Medicine, University of California Los Angeles, Los Angeles, California, USA;2. Leprosy Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil;3. Department of Dermatology, University of Southern California School of Medicine, Los Angeles, California, USA;4. Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA;1. Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan;2. Ph.D. Program for Aging, China Medical University, Taichung 40402, Taiwan;3. Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan;4. Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 70101, Taiwan;5. Department of Pharmacology, National Cheng Kung University, Tainan 70101, Taiwan;6. Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan |
| |
| Abstract: | The aim of this study was to investigate de novo bone formation following ectopic site implantation of bone substitutes covered by periosteum, with and without the application of autologous platelet-rich plasma (PRP). Twenty-four weeks after subcutaneous implantation of various bone substitutes (bovine hydroxyapatite (bHAP), phycogenic hydroxyapatite (pHAP), and bioglass (BG)) in 35 mini-pigs, bone regeneration rates were compared microradiographically and histologically. Without PRP, bHAP showed a mean de novo bone formation of 32.41% ± 29.99, in contrast to the other substitute materials where no mineralization could be detected. In combination with PRP, in the bHAP (63.61% ± 12.98; p ± 0.03) and pHAP (34.37 ± 29.38; p = 0.015) group, significantly higher de novo bone formation was ascertained than without PRP. No ossification could be detected in the BG group. In conclusion, bHAP and pHAP bone substitutes in combination with PRP showed a significant positive effect on periosteal cells by de novo bone formation after ectopic, subcutaneous, low-vascular site implantation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
