| Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease |
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| 作者姓名： | Zhi-Qin Mao Mei Sun |
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| 作者单位： | Zhi-Qin Mao，Mei Sun，Department of Pediatrics，Second Affiliated Hospital，China Medical University，Shenyang 110004，Liaoning Province，China Ying Huang，Hong Gao，Department of Pediatric Surgery，Second Affiliated Hospital，China Medical University，Shenyang 110004，Liaoning Province，China Qiang Ruan，Rong He，Ying Qi，Yu-Jing Huang，Yan-Ping Ma，Yao-Hua Ji，Zheng-Rong Sun，Virus Laboratory，Second Affiliated Hospital，China Medical University，Shenyang 110004，Liaoning Province，China |
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| 基金项目： | Supported by the National Natural Science Foundation of China, No.30170986 |
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| 摘 要： | AIM:To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants. METHODS:Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares,such as DNAclub,BioEdit,PROSITE database,and DNAstar. RESULTS:The strains from HD patients were distributed among three genotypes of UL144:group 1A (64%),group 2 (24%),and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (χ2 = 1.870,P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes. CONCLUSION:There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However,cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
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| 关 键 词： | Hirschsprung’s disease Cytomegalovirus UL144 gene Polymorphism |
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