白头翁醇提物对三硝基苯磺酸诱导大鼠结肠炎细胞免疫调节的研究

目的探讨白头翁醇提取物对三硝基苯磺酸（TNBS）诱导大鼠结肠炎的抗炎效果和对细胞免疫的调节。方法SD雄性大鼠28只，随机分成白头翁组（n-10），模型组（n-10），对照组（n-8）。白头翁组（n-10）和模型组建立TNBS大鼠结肠炎模型。白头翁组和模型组大鼠，用舍30mgTNBS的TNBS50％乙醇溶液0．85m1灌肠，对照组给予等量生理盐水灌肠。造模后白头翁组大鼠用白头翁醇提物灌肠，每天1次，模型组和对照组以等量生理盐水灌肠。造模后第8天断颈处死大鼠，进行疾病活动指数（DAI）和组织学损伤评分，ELISA法测定结肠组织中肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）水平。免疫组化学法检测结肠及脾脏组织CD4^＋T细胞、CD8^＋T细胞的表达。结果白头翁组DAI和组织学损伤评分较模型组低（P〈0．01）。结肠组织TNF-α、IL-6水平白头翁组低于模型组（P〈0．05或P〈0．01）。白头翁组结肠组织CD4^＋T细胞表达低于模型组（P〈0．05），而CD8^＋T细胞表达高于模型组（P〈0．05）。白头翁组脾脏组织CD8^＋T细胞表达高于模型组（P〈0．05）。结论白头翁醇提物对TNBS诱导大鼠结肠炎有抗炎作用，可能是通过对CD4^＋T、CD8^＋T细胞的免疫调节，促使细胞因子和炎症介质水平降低，而达到抗炎效果。

Effect of radix pulsatillae alcohol extract on colitis of rats induced by trinitrobenzenesulfonic acid

Objective To investigate the effect of radix pulsatillae alcohol extract on colitis of rats induced by trini- trobenzenesulfonic acid. Methods 28 male rats were randomly divided into radix pulsatillae alcohol extract treated group （group A, n=10）, TNBS control group （group B, n=10） and normal control group （group C, n= 8）. 0.85 ml enema containing 30 mg TNBS dissolved in 500% ethanol was instilled into the colon of the rats in group A and group B to induce distal colitis. The rats in group A were perfused through colon with radix pulsatillae alcohol extract at the dosage of 2g/ （kg · d） for 1 ml solution for 7 days, whereas the rats in group B and group C were treated with distilled water. The ani- mals were sacrificed at the 8th day of experiment. The disease activity index （DAD and the tissue damages of colonic mucosa were observed. The levels of tumor necrosis factor a （TNf-α） and interleukin-6 （IL-6） in colonic tissue were measured with enzyme-labeled immunosorbent assay （ELISA）. The expression of CD4^T cells and CD8^T cells in colonic tissue and spleen was detected with immunohistochemistry. Results Compared with group B, the disease activity index （DAD and histological damages of colonic mucosa in group A were significantly improved, the levels of TNF-α, IL-6 of colonic tissue and CD4^＋T lymphocytes in colonic tissue in group A were significantly decrease （P〈0.05）, and CD8^＋T lymphocytes in colonic tissue and spleen in group A were significantly increased （P〈0. 05）. Conclusion The radix palstillae alcohol extract demonstrated an anti-inflammatory action on colitis induced by trinitrobenzenesulfonic acid, and its mechanism might be involved in immunoregulating CD4^＋ and CD8^＋ T lymphocytes and reducing the levels of TNF-α and IL-6 in colonic tissue.