不同能量密度氦-氖激光照射对培养瘢痕成纤维细胞生长的影响

目的探讨氦-氖激光能量密度与培养瘢痕成纤维细胞生长的关系。方法以30、60、90、180和270J/cm2能量密度氦-氖激光(功率密度100mW/cm2),分别照射培养的人增生性瘢痕成纤维细胞1、3和5次,1次/d,然后采用台盼蓝染色法进行细胞计数。结果30J/cm2照射1次后细胞总数大于非照射组(P<0.001);90和180J/cm2照射3次,60、90和180J/cm2照射5次后细胞总数均少于同期非照射组(P<0.05),270J/cm2照射3、5次后细胞总数明显少于非照射组(P<0.001)。结论氦-氖激光对培养瘢痕成纤维细胞生长的抑制效应与能量密度、照射次数有关。

Effects of He-Ne laser with various energy densities on the growth of cultured fibroblasts in hypertrophic scar

Objective To explore the relationship of energy density of He Ne laser with the growth of fibroblasts in hypertrophic scar (HS).Methods The cultured fibroblasts in HS were irradiated with He Ne laser (power density 100 mW/cm2), various energy densities such as 30,60,90,180 and 270 J/cm2 were respectively adopted once a day.After 1,3 and 5 times of He Ne laser with each dose irradiation separately, the cell count was counted by trypan blue staining.Results The amount of cells after 1 times of 30 J/cm2 laser irradiation was markedly more than the control (P< 0.001).The amount of cells after 3 times of 90 and 180 J/cm2,5 times of 60 and 90 and 180 J/cm2 was less than the controls (P< 0.05).The amount of cells after 3 and 5 times of 270 J/cm2 was less than that controls (P< 0.001).Conclusion Inhibitory effect of He Ne laser irradiation on growth of scar fibroblasts is tightly associated with energy density and irradiation times.