米非司酮对绒癌细胞体外增殖及对非经典人类白细胞Ⅰ类抗原表达的影响

 目的 探讨米非司酮对绒癌细胞JEG 3体外增殖及对非经典人类白细胞I类抗原HLA G、HLA E表达的影响。方法 体外培养高表达HLA G、HLA E的绒癌细胞株JEG 3，采用MTT法检测米非司酮对细胞增殖的影响，分别通过RT PCR技术和流式细胞分析技术观察其对细胞中HLA G、HLA E mRNA和蛋白水平表达的影响。结果 米非司酮对JEG 3细胞的增殖表现出浓度信赖性的抑制作用，高浓度米非司酮能明显下调JEG 3细胞中HLA G、HLA E mRNA和蛋白水平。结论 米非司酮抗肿瘤的机制之一可能是其可以打破机体对肿瘤的免疫耐受，从而遏制肿瘤的生长。

Effect of Mifepristone on Proliferation and Expression of Non-classical Human Leucocyte Antigen ClassⅠMolecules in Choriocarcinoma Cell Line

Objective To explore the effect of mifepristone on the proliferation and the expression of nonclassical HLA I molecules (HLA G and HLA E) in choriocarcinoma cell line JEG 3.Methods The HLA G highly positive cell line of choriocarcinoma(JEG 3) was cultured in vitro,and MTT assay was used to examine antiproliferative effect of mifepristone on the JEG 3 cells,the mRNA expression of HLA G and HLA E were detected by RT PCR,and protein level by flow cytometry.Results Mifesitone produced concentration dependent and time dependent antiproliferative effect on JEG 3 cell at all experimental concentrations.High concentration mifesitone could significantly down regulate both mRNA and protein expression of HLA G and HLA E.Conclusion One of mifesitone’s antineoplastic mechanisms is surmounting immune tolerance to the neoplasm and inhibit its growth.Key words Mifesitone Human Leucocyte Antigen immune tolerance.