乌鲁木齐地区儿童人偏肺病毒感染状况 初步研究

目的 了解人偏肺病毒(hMPV)在乌鲁木齐地区儿童呼吸道感染患者中的致病情况.方法 选取2006年11月至2007年4月因呼吸道感染于新疆维吾尔自治区人民医院儿科住院患儿和部分门诊患儿的呼吸道分泌物及咽拭子标本共209份.通过反转录聚合酶链反应(RT-PCR)方法检测hMPV的M基因片段.并对阳性标本进行测序,用BLAST及DNAStar软件进行分析.结果 共检出2例阳性标本,占总例数1.0%.将2份阳性标本扩增的hMPV M基因片段进行对比,同源性为83.8%.进一步选取GenBank中与本次扩增目的基因相同的hMPV M基因片段做基因进化树分析,结果提示2例标本可能属于不同的2种基因型.结论 hMPV是乌鲁木齐地区儿童呼吸道感染病原之一,在一个流行季节当地可同时有2种不同基因型的hMPV流行.

Human metapneumovirus infection in children with acute respiratory tract inflammation in Urumuqi

OBJECTIVE: To understand whether human metapneumovirus (hMPV) is one of the pathogens leading to the children's respiratory infections in Urumqi. METHODS: A total number of 209 samples were collected in the People's General Hospital of Xinjiang Uygur Autonomous Region from November 2006 to April 2007 with some from the hospitalized children, while the others from outpatient clinic. Specimens included nasopharyngeal aspirates (NPA) and swabs were analyzed. Samples were all tested hMPV M gene by RT-PCR while the two positive PCR amplicons were sequenced and compared with other hMPV in GenBank by Blast and DNAstar. RESULTS: Of all the 209 samples, two positive ones were tested. The identities between them were 83.8%. Results from Phylogenetic analysis showed that they might belong to two different clusters. CONCLUSION: hMPV was one of the pathogens leading to the children's respiratory tract infections in Urumqi, with two different hMPV groups existed in the same season.