Fos蛋白和Jun蛋白在侧位液压冲击脑损伤大鼠大脑皮质的表达

目的：研究大鼠中度（０．２ＭＰａ）侧位液压冲击脑损伤时大脑皮质立即早期基因ｃ－ｆｏｓ和ｃ－ｊｕｎ表达产物Ｆｏｓ蛋白和Ｊｕｎ蛋白的变化规律。方法：雄性ＳＤ大鼠，随机分为正常对照物、手术对照组和损伤组。损伤组动物均给以０．２ＭＰａ液压冲击脑损伤，按冲击后处死时间不同再分为５、１５、３０、６０、１２０、２４０、４８０和７２０分钟组。应用免疫组织化学方法观察Ｆｏｓ和Ｊｕｎ蛋白在大脑皮质的表达特点。结果：冲击后３０分钟，双侧大脑皮质Ｆｏｓ阳性细胞数逐渐增多，冲击后７２０分钟达高峰。Ｆｏｓ阳性细胞面积在冲击后１２０分钟逐渐增大，７２０分钟达高峰；冲击后６０分钟双侧大脑皮质Ｊｕｎ阳性细胞数逐渐增多。冲击后１２０分钟阳性细胞面积逐渐增大，冲击后７２０分钟阳性细胞数和面积均达高峰。结论：中度侧位液压冲击脑损伤后Ｆｏｓ蛋白和Ｊｕｎ

Expression of c-fos and c-jun in cerebral cortex following moderate lateral hydraulic percussion brain injury in rats

Objective Toinvestigate the protein expression of cfos and cjun in cerebral cortex following moderatelateral fluid percussion brain injury(0. 2 MPa) in rats.MethodsMale SpragueDawley ratswere divided into normal control,sham o peration control and injury group.Animals ininjury group were subjected to a m oderate lateral hydraulic percussion brain injury (0.2MPa),and then were subdi vided into 5,15,30,60,120,240,480 and 720 minutes groupsaccording to the tim e elapsed after injury.The expression of cfos and cjun w asdetermined by immunohistochemistry. ResultsThirty minutes after percussion,Fos positiveneurons increase d bilaterally and progressively in cerebral cortex,peaking at 720minutes.The a rea of Fos positive neurons elevated at 120 minutes after percussion,peakingat 720 minutes.Jun positive neurons increased bilaterally at 60 minutes after percussion,and remained elevated up to 720 minutes after brain injury.The area of Jun positiveneurons increased at 120 minutes af ter percussion,peaking at 720 minutes. ConclusionsTheinduction and expression of cf os and cjun in cerebral cortex increased bilaterally aftermoderate hydraulic percussio n brain injury,and lasted for a long period.The elevatedinduction of Jun appea rs to be delayed as compared with Fos,but the temporal pattern andexpression e xtent might be similar to that of Fos.