Physiological properties of retinal Muller glial cells from the cynomolgus monkey, Macaca fascicularis--a comparison to human Muller cells |
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Authors: | Pannicke Thomas Biedermann Bernd Uckermann Ortrud Weick Michael Bringmann Andreas Wolf Sebastian Wiedemann Peter Habermann Gunnar Buse Eberhard Reichenbach Andreas |
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Affiliation: | Paul-Flechsig-Institut für Hirnforschung, Universit?t Leipzig, Jahnallee 59, D-04109 Leipzig, Germany. pant@medizin.uni-leipzig.de |
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Abstract: | Retinae from rabbits and laboratory rodents are often used as 'models' of the human retina, although there are anatomical differences. To test whether monkey eyes provide a better model, a physiological study of Muller glial cells was performed comparing isolated cells and retinal wholemounts from the cynomolgus monkey, Macaca fascicularis and from man. The membrane conductance of Muller cells from both species was dominated by inward and outward K(+) currents. Cells displayed glutamate uptake currents and responded to nucleotides by intracellular Ca(2+) increases. However, there were also species differences, such as a lack of GABA(A) receptors and of Ca(2+)-dependent K(+) currents in monkey cells. Thus, the use of Muller cells from cynomolgus monkeys may be advantageous for investigating a few specific properties; in general, monkey cells are no more similar to human cells than those from standard laboratory animals. |
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Keywords: | Patch-clamp methodology Ca2+ microfluorimetry K+ currents Membrane channels P2 receptors |
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