| 永生化人晶状体上皮细胞的体外培养 |
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| 引用本文： | 胡俊,黄秀榕,祁明信,杨丽英. 永生化人晶状体上皮细胞的体外培养[J]. 微量元素与健康研究, 2010, 27(5): 4-6 |
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| 作者姓名： | 胡俊 黄秀榕 祁明信 杨丽英 |
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| 作者单位： | 解放军第180医院眼科医院;福建中医药大学病理生理研究中心;福建省第二人民医院眼科; |
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| 基金项目： | 福建省卫生厅重点资助项目 |
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| 摘 要： | 目的:建立永生化人晶状体上皮细胞(Human lens epithelial cell,HLEC)培养、冻存与复苏技术。方法:传代培养人晶状体上皮细胞系B-3,并进行细胞冻存与复苏,台盼蓝染色法判断细胞活力。结果:接种后的HLEC在短时间内即可贴壁及延伸,12～24 h后大部分细胞贴壁生长,具有上皮细胞的形态特点,约3～4 d细胞基本融合。冻存后复苏的HLEC活力超过90%,保持正常的细胞形态,并可长期传代培养。结论:成功建立永生化HLEC培养、冻存与复苏技术,为白内障相关研究提供理想的细胞材料。
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| 关 键 词： | 晶状体上皮细胞 细胞系 细胞培养 白内障 |
Culture system in vitro of immortalized human lens epithelial cell |
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| HU Jun,HUANG Xiu-rong,Qi Ming-xin,et al. Culture system in vitro of immortalized human lens epithelial cell[J]. Studies of Trace Elements and Health, 2010, 27(5): 4-6 |
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| Authors: | HU Jun HUANG Xiu-rong Qi Ming-xin et al |
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| Affiliation: | HU Jun~1,HUANG Xiu-rong~2,Qi Ming-xin~3,et al (1.The Department of Ophthalmology in Liberation Army 180 th Hospital,Quanzhou 362000,2.The Research Center of Pathophysiology,Fujian College of Traditional Chinese Medicine,3.The affiliated Second Peoples Hospital,Fujian College of Traditional Chinese Medicine:Fuzhou 350003,China) |
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| Abstract: | Objective:To establish the subculture,cryopreservation and anabiosis technique of immortalized human lens epithelial B- 3 (HLE B - 3) cell.Methods:HLE B - 3 cell was cultured,frozen and resuscitated in vitro.The cell activity was determined by trypan -blue dye and the morphology of HLE B - 3 cell was observed.Results:After 12 to 24 hours the cells began to grow adhesively,and showed the typical morphological features of epithelia,the cells ran together primitively after 3 to 4 days.the viability of HLE B - ... |
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| Keywords: | Lens epithelial cell Cell line Cell culture Cataract |
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