| 七氟醚对吸入麻醉药敏感性不同的果蝇幼虫中枢神经元钾通道的影响 |
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| 引用本文： | 刘洋,廖大清,罗南富,刘进.七氟醚对吸入麻醉药敏感性不同的果蝇幼虫中枢神经元钾通道的影响[J].临床麻醉学杂志,2010,26(2). |
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| 作者姓名： | 刘洋 廖大清 罗南富 刘进 |
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| 作者单位： | 1. 川北医学院附属医院麻醉科,四川省南充市,637000
2. 四川大学华西医院麻醉与危重急救研究室 |
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| 基金项目： | 国家自然科学基金(30070732);;教育部博士点基金 |
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| 摘 要： | 目的以钾通道为切入点,利用膜片钳技术探讨吸入麻醉药的作用机制。方法分离制备对吸入麻醉药敏感(S)、耐药(R)和野生(H)果蝇品系脑神经元,以膜片钳技术观察临床有效浓度七氟醚对其全细胞钾电流的影响。结果1MAC七氟醚使三品系Ⅲ型钾电流的Ipeak值显著增加:S(19±2)%>H(13±2)%>R(8±2)%;使钾电流的恢复曲线显著上移;不改变钾离子的平衡电位。2MAC七氟醚使三品系Ⅲ型钾电流的Ipeak值显著增加:S(36±4)%>H(24±2)%>R(14±1)%。3MAC的七氟醚能使Ⅲ型钾电流稳态失活曲线显著右移。结论钾离子通道可能是吸入麻醉药的中枢作用位点之一。
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| 关 键 词： | 七氟醚 果蝇 钾电流 |
Effects of sevoflurane on currents in the larval cerebral neurons of Drosophila with different sensitivity to volatile anesthetics |
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| LIU Yang,LIAO Da-qing,LUO Nan-fu,et al..Effects of sevoflurane on currents in the larval cerebral neurons of Drosophila with different sensitivity to volatile anesthetics[J].The Journal of Clinical Anesthesiology,2010,26(2). |
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| Authors: | LIU Yang LIAO Da-qing LUO Nan-fu |
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| Institution: | LIU Yang,LIAO Da-qing,LUO Nan-fu,et al. Department of Anesthesiology,North Sichuan Medical College,Nanchong 637000,China |
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| Abstract: | Objective Drosophila was applied as animal model to investigate K+ channels targets for volatile anesthetics with voltage-clamp recording technique.Methods Three strains of Drosophila with high (S),normal (H) and low (R) sensitivity to sevoflurane were developed by 33 generations' screening selection. Cerebral neurons from the three strains of Drosophila were obtained by acutely enzymatic digestion and incubation in fetal bovine serum. Whole-cell potassium currents were recorded using voltage-clamp recordin... |
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| Keywords: | Sevoflurane Drosophila melanogaster K+ currents |
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