| 护津方对急性肺损伤大鼠肺组织金属基质蛋白酶2mRNA及其抑制因子2mRNA表达的影响 |
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| 引用本文： | 杨爱东,汪东颖,李文雯,吴中华,郭永洁,庞慧芳,符胜光. 护津方对急性肺损伤大鼠肺组织金属基质蛋白酶2mRNA及其抑制因子2mRNA表达的影响[J]. 中国中医药科技, 2010, 17(2): 98-99 |
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| 作者姓名： | 杨爱东 汪东颖 李文雯 吴中华 郭永洁 庞慧芳 符胜光 |
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| 作者单位： | 上海中医药大学,上海,201203 |
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| 基金项目： | 上海市教委高校高水平特色发展项目No.沪教委财(2005)81号，上海中医药大学名师传承工程研究项目No.2005-62 |
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| 摘 要： | 目的：探讨护津方对细菌脂多糖诱导的急性肺损伤（Au）大鼠肺组织金属基质蛋白酶2mRNA（MMP一2mRNA）及其抑制因子2mRNA（tiMP一2mRNA）表达的影响。方法：40只雄性Wislar大鼠随机分为4组：正常对照组、LPS模型组、地塞米松组和护津方组，每组再分为4小时和8小时两个亚组。分别用地塞米松和护津方在LPS诱导Au模型前预处理大鼠。检测大鼠肺体指数，实时荧光定量PcR检测肺组织MMP一2mRNA、TIMP一2mRNA表达，并光镜观察肺组织病理形态变化。结果：与模型组相比，在两个时相点护津方组大鼠肺体指数显著降低（P〈0．01或P〈0．05）、MMP一2mRNA表达表达明显降低（P〈0．01）及TIMP一2mRNA表达明显升高（P〈0．01或P〈0．05）。病理学观察，模型组大鼠肺组织出现大片出血及坏死，护津方组大鼠肺组织病理损伤程度较模型组减轻。结论：护津方对内毒素致急性肺损伤大鼠有保护作用，其机理可能与其能降低MMP一2mRNA的表达和升高TIMP一2mRNA的表达有关。
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| 关 键 词： | 肺损伤／中医药疗法 @护津方／治疗应用 肺损伤／病理学 基因表达 大鼠 |
EFFECTS OF HUJIN DECOCTION ON MMP-2mRNA AND TIMP-2mRNA EXPRESSION IN LUNGS OF RATS WITH ACUTE LUNG INJURY |
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| YANG Ai-dong,WANG Dong-ying,LI Wen-wen,et al. EFFECTS OF HUJIN DECOCTION ON MMP-2mRNA AND TIMP-2mRNA EXPRESSION IN LUNGS OF RATS WITH ACUTE LUNG INJURY[J]. Chinese Journal of Traditional Medical Science and Technology, 2010, 17(2): 98-99 |
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| Authors: | YANG Ai-dong WANG Dong-ying LI Wen-wen et al |
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| Affiliation: | YANG Ai-dong,WANG Dong-ying,LI Wen-wen,et al(Shanghai University of Traditional Chinese Medicine,Shanghai201203) |
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| Abstract: | Objective:To discuss the effects of hujin decoction on expression of metalloproteinase-2mRNA(MMP-2mRNA)and tissue inhibitor of metalloproteinase-2mRNA(TIMP-2mRNA)of lungs of rats with acute lung injury caused by LPS.Methods:40 rats were divided into 4 groups,normal,model,dexamethasone and hujin decoction groups,each group was divided into two subgroups after LPS-induced 4 and 8 hours,dexamethasone and hujin decoction groups were respectively given dexamethasone and hujin decoction before injecting LPS to in... |
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| Keywords: | Hujin decoction could reduce acute lung injury induced by LPS which was related to decreasing the expression of MMP-2mRNA and increasing the expression of TIMP-2mRNA. |
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