血管紧张素转换酶和内皮型一氧化氮合酶基因多态性与高血压的相关性

目的探讨血管紧张素转换酶(ACE)基因I/D、内皮型一氧化氮合酶 (eNOS)基因G894T多态性与原发性高血压(EH)的关系.方法采用基因芯片技术检测 224例(124例高血压患者和100例健康人)ACE和eNOS基因多态性.结果 EH组ACE DD基因型携带者频率显著高于对照组 (30.65% vs16.00%,P<0.05 ),eNOS- GT TT基因型频率与对照组相比无显著性差异( 12.90% vs 15.00%,P＞0.0 5).结论 ACE-DD基因多态性可能是高血压病的独立危险因素,eNOS基因G894T多态性可能不是高血压病的独立危险因素.基因芯片技术为研究多种易感基因与高血压病的相关性提供了一项高效、敏感的方法.

The relationship of angiotensin-converting enzyme and endothelial nitric oxide synthase gene polymorphisms In predisposition to essential hypertension

Objective To evaluate the involvement of Angiotensin-converting enzyme(ACE) and endothelial nitric oxide synthase(eNOS) gene polymorphisms in the development of essential hypertension(EH).Methods The study included 224 subjects(124 with EH and 100 controls).Gene chip technology was performed.The allele frequency and genotype distribution were compared between groups.Results A significantly higher frequency of the DD genotype of ACE gene was observed in EH group(30.65% vs16.00%,P<0.05).The ACE gene polymorphism was associated with EH,No statistically significant difference was observed in eNOS GT+TT genotypes distribution between the two groups(12.90%vs15.00%,P>0.05).Conclusion The ACE but not eNOS gene polymorphism may be an independent risk factor of EH in China.Gene chip technology provides an effective and sensitive method to study the relationship of gene polymorphisms and EH.