| 肿瘤坏死因子相关凋亡诱导配体诱导骨肉瘤细胞凋亡的研究 |
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| 引用本文： | 黄涛,吕刚,李楠,王岩峰,安贵峰,于德水,马旭. 肿瘤坏死因子相关凋亡诱导配体诱导骨肉瘤细胞凋亡的研究[J]. 中国医科大学学报, 2006, 35(2): 175-176 |
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| 作者姓名： | 黄涛 吕刚 李楠 王岩峰 安贵峰 于德水 马旭 |
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| 作者单位： | 1. 中国医科大学附属第一医院骨科,辽宁,沈阳,110001
2. 中国医科大学第87期7年制 |
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| 摘 要： | 目的：研究肿瘤坏死因子相关凋亡诱导配体（TRAIL）对骨肉瘤OS-732细胞的诱导凋亡作用，探寻骨肉瘤临床化疗的斯方案。方法：将TRAIL应用于体外培养的骨肉瘤OS-732细胞，采用MTr法检测细胞毒性作用，倒置相差显微镜及荧光染色方法观察肿瘤细胞形态改变，扫描及透射电镜在亚细胞形态上证实凋亡细胞。结果：不同浓度TRAIL作用下，细胞抑制率问差别十分显著（P〈0.01）。超微结构观察显示骨肉瘤细胞凋亡。结论：TRAIL诱导骨肉瘤细胞凋亡，且具有浓度依赖性。
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| 关 键 词： | 肿瘤坏死因子相关凋亡诱导配体 骨肉瘤 凋亡 |
| 文章编号： | 0258-4646(2006)02-0175-02 |
| 收稿时间： | 2005-04-06 |
| 修稿时间： | 2004-09-27 |
Experimental study on apoptosis of osteosarcoma cells induced by TNF-related apoptosis-inducing ligand |
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| HUANG Tao,L Gang,LI Nan,WANG Yan-feng,AN Gui-feng,YU De-shui,MA Xu. Experimental study on apoptosis of osteosarcoma cells induced by TNF-related apoptosis-inducing ligand[J]. Journal of China Medical University, 2006, 35(2): 175-176 |
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| Authors: | HUANG Tao L Gang LI Nan WANG Yan-feng AN Gui-feng YU De-shui MA Xu |
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| Affiliation: | 1. Department of Orthopedics, The First Affiliated Hospital, China Medical University, Shenyang 110001, China; 2. The 87 Grade, Seven Year System |
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| Abstract: | Objective: To study the role of TNF-related apoptosis-inducing ligand(TRAIL) in the apoptosis of osteosarcoma cell line(OS-732) and explore the new chemotherapeutic strategy.Methods: TRAIL was administered to OS-732 cells cultured in vitro,and the inhibition rate of OS-732 cells was measured by using MTT assay.The cellular ultrastructure was observed under inverted phase-contrast microscope,fluorescence microscope,and scanning and transmission electron microscope.Results: There was significant difference in the inhibition rate among OS-732 cells under different concentrations of TRAIL 24 hours after administration of TRAIL(P<0.01).The changes of cellular ultrastructure showed TRAIL induced the apoptosis of OS-732 cells.Conclusion: TRAIL could induce the apoptosis of osteosarcoma cells in a dose-dependent manner. |
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| Keywords: | TNF-related apoptosis-inducing ligand osteosarcoma apoptosis |
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