| 人源蓝氏贾第虫病毒GLV1518-2322基因的表达及表达产物抗血清的制备 |
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| 引用本文： | 田宗成,张西臣,李建华,尹继刚,杨举. 人源蓝氏贾第虫病毒GLV1518-2322基因的表达及表达产物抗血清的制备[J]. 寄生虫与医学昆虫学报, 2004, 11(1): 5-7 |
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| 作者姓名： | 田宗成 张西臣 李建华 尹继刚 杨举 |
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| 作者单位： | 解放军军需大学军事兽医系预防兽医学教研室,吉林,长春,130062;解放军军需大学军事兽医系预防兽医学教研室,吉林,长春,130062;解放军军需大学军事兽医系预防兽医学教研室,吉林,长春,130062;解放军军需大学军事兽医系预防兽医学教研室,吉林,长春,130062;解放军军需大学军事兽医系预防兽医学教研室,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 960 0 110 )，总后勤部留学回国人员基金资助项目 ( 98H0 2 5 ) |
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| 摘 要： | 根据中国人源蓝氏贾第虫病毒 (GLV)全基因组测序结果 ,将其全基因组cDNA上编码贾第虫病毒部分衣壳蛋白的GLV1 5 1 8 2 3 2 2基因克隆到原核表达载体pET 2 8c ( +)上 ,构建了原核表达重组质粒pET 2 8c( +) GLV1 5 1 8 2 3 2 2。SDS PAGE分析表明 ,经IPTG诱导 ,3 2kDa蛋白基因在大肠杆菌BL2 1 (DE3 )pLysS中得到高效表达。以纯化的表达产物为抗原 ,免疫BalB c小鼠 ,同时用病毒粒子免疫BalB c小鼠 ,制备了中国人源蓝氏贾第虫病毒GLV1 5 1 8 2 3 2 2蛋白特异性抗血清 ,ELISA方法测得的纯化蛋白最适抗原包被浓度为1 40 0和病毒粒子抗血清最佳工作浓度为 1 2 0 0。
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| 关 键 词： | 贾第虫病毒 蓝氏贾第虫 GLV1518-2322 |
| 修稿时间： | 2003-04-03 |
THE EXPRESSION OF GLV1518-2322 GENE FROM GIARDIAVIRUS IN GIARDIA LAMBLIA ISOLATED FROM HUMAN IN CHINA AND THE PREPARATION OF ITS ANTIBODIES |
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| TIAN Zong-Cheng ZHANG Xi-Chen LI Jian-Hua YIN Ji-Gang YANG Ju. THE EXPRESSION OF GLV1518-2322 GENE FROM GIARDIAVIRUS IN GIARDIA LAMBLIA ISOLATED FROM HUMAN IN CHINA AND THE PREPARATION OF ITS ANTIBODIES[J]. Acta Parasitologica et Medica Entomologica Sinica, 2004, 11(1): 5-7 |
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| Authors: | TIAN Zong-Cheng ZHANG Xi-Chen LI Jian-Hua YIN Ji-Gang YANG Ju |
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| Abstract: | According to the sequence of Giardiavirus genome from China,the recombinant plasmid pET-28c(+)-GLV1515-2322 coding part of the coat protein of Giardiavirus was developed.After it was transformed into E.coli strain DE3,then induced by IPTG,a 32kDa expression protein was found by SDS-PAGE.After Balb/c mice were immunized with the expression protein,antiserum of GLV1518-2322 was prepared. |
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| Keywords: | Giardia lamblia Giardiavirus GLV1518-2322 |
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