Her-2/neu、DPC4和P16在胰腺癌中的表达及其意义

目的观察Her-2/neu、P16和DPC4基因在胰腺癌中的表达,并探讨其与胰腺癌发生、发展的关系。方法应用免疫组织化学法检测Her-2/neu、P16和DPC4基因在34例胰腺癌和12例胰腺良性病变和正常组织中的表达水平,将结果与患者的临床病理学资料进行统计。结果胰腺癌Her-2/neu表达水平和P16表达缺失水平显著高于胰腺良性病变和正常组织(P<0.05);胰腺癌DPC4表达水平低于胰腺良性病变和正常组织。高、中度分化胰腺癌DPC4表达水平显著高于低分化胰腺癌(P<0.05);有淋巴结转移的胰腺癌Her-2/neu和P16表达水平显著低于无淋巴结转移的胰腺癌(P<0.05);随TNM分期增高,Her-2/neu表达阳性率显著降低(Ⅰ期85.7%;Ⅱ期54.5%,Ⅲ期36.4%,Ⅳ期0.0%)(P<0.05);P16表达阳性率亦显著降低(Ⅰ期100.0%;Ⅱ期72.7%,Ⅲ期54.5%,Ⅳ期20.0%)(P<0.05)。Her-2/neu与P16和DPC4与Her-2/neu的表达密切相关(P<0.05);P16与DPC4的表达极密切相关(P<0.01)。结论Her-2/neu、P16和DPC4的表达对估计胰腺癌的生物学行为和判断预后有较大帮助。P16和DPC4的表达对胰腺癌的进展可能有协同作用。

Expression of Her-2/neu, DPC4 and P16 in pancreatic carcinoma and its implication

Objective To detect the expression of genes Her-2/neu,DPC4 and P16 in pancreatic carcinomas and to investigate the role of their alterations in tumorigenesis and progression of pancreatic carcinomas.Methods We studied the immunohistochemical markers Her-2/neu,DPC4 and P16 in 34 adenocarcinomas and 12 nonmalignant specimens of the pancreas,and the relationship between DPC4 alterations and various clinicopathological parameters was evaluated.Results There was a significant difference between normal pancreatic tissues and benign pancreatic lesions and primary pancreatic carcinomas for frequency of Her-2/neu expression and loss of P16 expression(P<0.05).The level of loss of DPC4 expression was increased in primary pancreatic carcinoma in comparison to nonmalignant specimens of the pancreas.The frequency of loss of DPC4 expression was greater in primary pancreatic carcinomas with poor-differentiation than with good and moderate-differentiation(P<0.05).The frequency of Her-2/neu expression and loss of P16 expression were greater in primary pancreatic carcinomas with lymph node-positive than with lymph node-negative(P<0.05);Followed in TNM staging,the level of Her-2/neu expression was significantly decreased(P<0.05),and the frequency of loss of P16 expression was also significantly increased(P<0.05).The expression of Her-2/neu was correlated with P16(P<0.05);the expression of Her-2/neu was correlated with DPC4(P<0.05);the expression of P16 was correlated with DPC4(P<0.01).ConclusionThese data suggest that the abnormal expression of Her-2/neu,DPC4 and P16 was potential marker for predicting the prognosis.The expression of P16 and DPC4 has an impact on the development of pancreatic carcinoma.