宁血丸对ITP模型小鼠外周血小板的影响

目的观察ITP小鼠模型外周血血小板数量与功能的变化情况，探讨宁血丸改善ITP的效果。方法采用全自动血细胞分析仪检测外周血血小板计数，采用流式细胞仪检测CIMI（抗GPⅢ3a）、CD61（抗GPⅡb）。结果注射抗血小板血清（APS）后ITP模型小鼠外周血血小板计数明显下降，差异有显著性意义（P〈0．001）；宁血丸能显著提升外周血血小板计数；可使CIM-1、CD61上升并接近正常水平。结论ITP的存在使血小板数量减少及功能异常；中药宁血丸可明显提高外周血血小板的数量，还可提高血小板的聚集功能。

Influence of Ningxuewan Granules on the Peripheral Blood Platelets in Mouse Model of Idiopathic Thrombocytopenic Purpura

Objective To investigate the changes of peripheral blood platelet count and function in the mouse model of idiopathic thrombocytopenic purpura(ITP) and to evaluate the therapeutic effect of a Chinese medicine Ningxuewan(NXW) on those changes.Methods The peripheral platelet count was conducted with an automatic hemocyte analyzer.CD41 and CD61 level was determined by flow cytometry. Results The amount of the peripheral blood platelets in ITP model mice was decreased after injection of antiplatelet serum(APS) and CD41 and CD61 were also decreased as compared with those before injection(P<0.001). NXW in high-dose increased the amount of platelets,and elevated the content of CD41 and CD61 to a normal level.Conclusion The platelet count and function are abnormal in mice in the episode of ITP.NXW can increase the amount of peripheral blood platelets and restore the aggregation function of platelets.