甘遂半夏汤对正常大鼠一般情况及心率、肾上腺的影响

目的: 探讨甘遂半夏汤水煎液对正常大鼠一般情况、心率、皮质醇水平及肾上腺形态的影响。方法: 将80只SD大鼠随机分为甘遂半夏汤高、中、低剂量组(37,18.5,9.25 g·kg^-1)及正常组,各组大鼠采用等体积不等浓度ig,ig体积均为0.02 mL·g^-1。给药组ig给药,正常组ig给蒸馏水。给药90 d。每日给药后观察并记录大鼠的行为、活动、饮食、睡眠、毛色、粪便等变化。实验前、后各称量体重、计数心率1次。ig期间每周称重2次。最后1次给药后禁食15 h,取血,检测皮质醇;取肾上腺,观察大体形态和组织学形态。结果: 在给药期,与正常组比较,甘遂半夏汤各剂量组大鼠体重、心率及皮质醇均无显著差异。大鼠的行为、活动、饮食、睡眠、毛色、粪便及肾上腺形态无明显异常。结论: 长期服用甘遂半夏汤水煎液对大鼠一般情况、心率、皮质醇及肾上腺形态无显著影响。

Effect of Gansui Banxia Tang on the General Situation in Normal Rats

Objective: To explore the chronic toxicity of Gansui Banxia Tang (GBT) on the general situation, heart rate, cortisol level and adrenal gland morphology in normal rats. Method: Eighty healthy SD rats were randomly divided into four group:the normal group, the high-, middle-, and low-dose GBT groups (37, 18.5 and 9.25 g·kg^-1). The corresponding medicines at 0.02 mL·g^-1were intragastrically administrated to rats for 90 days.The general situation changes including action, activity, diet, sleep, color, excrement and urine were observed once daily after administration. The body weight was observed twice weekly and the heart rate was recorded once before and once after the experiment. After the last dose, the blood samples were collected and the morphology and histology of the adrenal gland were observed by HE staining. Result: In medicine taking, no significant differences for the change of body weight, heart rate and cortisol were found between any two groups. Moreover, there were no obvious changes for the general situation changes including action, activity, diet, sleep, color, excrement, urine and adrenal gland morphology. Conclusion: GBT has no remarkable effects on the general situation, heart rate, cortisol level and adrenal gland morphology in normal rats by long-term medication.