血管抑素抑制大鼠角膜新生血管的研究

目的研究血管抑素（AS）对大鼠角膜碱烧伤后新生血管的抑制作用。方法120只Wistar大鼠制作碱烧伤角膜新生血管（CNV）模型,随机分为4组,分别为2.5μgAS组、5μgAS组、地塞米松组、生理盐水组,每组30只。分别给予2.5μg/0.1mLAS、5μg/0.1mLAS、0.1mg/0.1mL地塞米松、生理盐水各0.1mL,球结膜下注射,隔日1次,共4次。在大鼠角膜碱烧伤后不同时间裂隙灯下观察大鼠角膜混浊度,计算新生血管面积,分析角膜组织病理切片。结果2.5μgAS组、5μgAS组及地塞米松组在第7、10、14天较生理盐水组角膜混浊程度轻,差异均有统计学意义（P〈0.05）;2.5μgAS组、5μgAS组及地塞米松组在碱烧伤后第3天起各时间点新生血管面积小于生理盐水组,差异均有统计学意义（P〈0.05）。结论AS能有效抑制大鼠角膜碱烧伤后CNV的形成。

Inhibitory effect of angiostatin on rat corneal neovascularization

Objective Angiostatin is thought to be one of the most effective neovascularization inhibitors. Many researches have revealed the strong inhibting effect on tumour neovascularization. But seldom report is published in the study of effect of angiostatin on corneal neovaseularization （CNVs） owing to alkali burn. Present study was to investigate the inhibitory effect of angiostatin on corneal neovaseularization caused by alkali burn. Methods Corneal alkali-burned model was established in 120 Wistar rats by placing the filter paper soaked 1 mol/L NaOH solution at the center of cornea. The rat models were divided into four groups at random and 30 rats for each. 2.5 μg/0. 1 mL angiostatin,5 μg/0. 1 mL angiostatin,0.1 mg/0. 1 mL dexamethasone and 0.1 mL 0. 9% saline solution was subconjunctivally injected respectively in different groups for 4 times during the one week duration. Corneal opacity of rats was examined and scored by slit-lamp microscope at different time points. The new blood vessel area in corneas were calculated on the formation of S = C/12 × 3. 141 6 [ r2 - （ r - L） 2 ]. The pathological sections of cornea were analyzed at 1,3,7,10,14,21 days after injection under the light electron microscope. The use of animal followed the Standard of Association for Research in Vision and Ophthalmology. Results The corneal opacity score in 2.5 μg/0.1 mL angiostatin, 5 μg/0.1 mL angiostatin,0. 1 mg/0. 1 mL dexamethasone group was lower than that in 0. 9% saline solution group at 7,10,14 days after cautery with statistically significant differences（ P 〈 0.01 ）. On the 14th day after alkali burned, CNVs presented the densely distribution over the cornea in 0.9% saline solution group, but a fewer CNVs were seen in 5 μg/0. 1 mL angiostatin group. The new blood vessels buds were seen, but fewer mature vessel formed in 5 μg/0. I mL angiostatin group. However, a lots of mature vessels with mature erythrocytes in 0.9% saline solution group on the 14th day after alkali burned. Conclusion Angiostatin can eff