脑梗死后神经细胞黏附分子的动态变化及滋补肝肾复方复健片的干预效应

目的]观察复健片在不同时间点对脑梗死大鼠脑组织神经细胞黏附分子表达的影响,探讨其促进神经功能恢复的作用机制。方法]240只Wistar大鼠随机分为正常对照组、假手术组、模型组和药物组共4个大组,并根据脑梗死病程每组又分为3、7、14、28、42d共5个亚组,每个亚组动物数为12只。用改良的LongaEZ法制备大脑中动脉阻塞大鼠模型,药物组灌胃给予复健片水溶液,余各组分别灌胃给予同剂量的蒸馏水。用免疫组织化学法观察模型大鼠梗死周边区脑组织神经细胞黏附分子的表达。结论]模型组大鼠脑梗死后脑内的神经细胞黏附分子(NCAM)表达逐渐增高,在28d时达到高峰,后逐渐下降,其与正常对照组各时相比较有非常显著性差异(P<0.01)。药物组在3d时出现高表达,14d时其表达达到高峰,持续至28d时开始下降,药物组的NCAM表达与模型组同时间点比较有非常显著性差异(P<0.01)。结论]复健片可增加NCAM的表达,有助于轴突生长和靶向迁移,促进神经功能恢复。

Change of expression of NCAM in MCAO rats at the different stages and effect of Chinese medicine Fujian Tablet with nourishing the liver and kidney on NCAM

Objective] To explore the effective mechanism of Fujian Tablet promoting neural regeneration by observing the effect of Fujian tablet on the expression of NCAM in MCAO rats at the different stages. Methods] Two hundred and forty Wistar rats were randomly divided into normal control, sham operation, model and medicine groups. They were subsequently divided into five subsets with 12 rats in each group according to the disease course at 3, 7, 14, 28 and 42 days. The rat models with middle cerebral artery occlusion were successfully established by the improved Longa EZ. The medicine group were given with Fujian Tablet, other group with commensurability distilled water. The expression of NCAM in the region around the infarction was observed by immunohistochemistry method. Results] In the model group, NCAM-positive cells gradually increased and reached a peak at day 28, and then decreased, which were notably different from normal control group (P<0.01). In the medicine group, NCAM-positive cells were obviously different with normal control group (P<0.01), obviously increased at day 3, and reached a peak at day 14, then still remained high level, decreased from day 28, but were still obviously different compared with model group (P<0.01). Conclusion] The Fujian Tablet can increase the NCAM positive cells, and promote axon growth and target migration.