活血化瘀注射液(HHI-I)对TNF-α诱发大鼠肺微血管内皮细胞黏附分子mRNA表达的影响

目的]从调节白细胞内皮细胞过度黏附角度研究活血化瘀中药的作用机制。方法]体外培养肺微血管内皮细胞,RT-PCR方法观察活血化瘀注射液对肿瘤坏死因子-α(TNF-α)诱发的体外培养大鼠肺微血管内皮细胞E-选择素、P-选择素、细胞间黏附分子ICAM-1mRNA表达的影响。结果]肺微血管内皮细胞与含TNF-α培养基共同孵育后,可显著增加内皮细胞E-选择素、P-选择素、ICAM-1mRNA表达,在培养基中加入活血化瘀注射液含药血清后,则内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达被明显抑制。结论]抑制内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达是活血化瘀重要的作用机制之一。

Effect of HHI-I on TNF-alpha Mediated Expression of Adhesion Molecules mRNA of Pulmonary Microvascular Endothelium in Rats

Objective] To elucidate the mechanism underlying the effect of Huoxue Huayu(Activating blood circulation and remove stasis) herbal medicine on the recruitment of leukocytes in blood circulation. Methods] The effect of HHI-I(Huoxue Huayu Injection No.I) on the expression of adhesion molecules mRNA induced by TNF-alpha was investigated in vitro with RT-PCR technique. Results] In the cultured lung microvascular endothelial cells stimulated with TNF-alpha, the expression of adhesion molecules, such as E-selectin, P-selectin, ICAM-1 mRNA, was increased significantly. In contrast, the cells exposed to HHI-I simultan eously exhibited a marked lower expression of the TNF-alpha induced endothelial adhesion molecules. Conclusion] HHI-I can inhibit the excessive expression of endothelial cell adhesion molecules.