裸鼠肿瘤动物模型VEGF受体表达及其意义

目的　通过免疫组织化学染色了解flt 1与flk 1 KDR(VEGF的两个高亲和受体 )在人肿瘤细胞皮下接种肿瘤动物模型的血管内皮细胞与肿瘤细胞中的表达。方法　取荷瘤裸鼠皮下接种瘤块 ,漂洗、固定、石蜡连续切片 ,进行两种受体相应免疫组化检测。结果　在 13种荷瘤裸鼠血管内皮细胞及肿瘤细胞中flt 1的阳性率大部分为强阳性或中阳性 ,而只有在荷人胃腺癌MKN 4 5裸鼠的肿瘤细胞中flt 1的阳性率为弱阳性 ,在荷人卵巢癌SKOv3裸鼠的肿瘤细胞中flt 1的表达为阴性。相比较而言 ,在 13种荷瘤裸鼠血管内皮细胞及肿瘤细胞中KDR的阳性率大部分为中阳性或弱阳性 ,并且在荷人肝癌SMMC 772 1裸鼠 ,荷人胃腺癌SPC A1裸鼠 ,荷人高转移肝癌移植瘤裸鼠 ,荷人卵巢癌SKOv3裸鼠的肿瘤细胞中 ,荷人宫颈癌移植瘤裸鼠和荷人胃腺癌MKN 4 5裸鼠的肿瘤细胞中 ,KDR表达为阴性。结论　VEGF受体共同表达于肿瘤血管内皮细胞与肿瘤细胞 ,提示了VEGF与VEGF受体结合作用在肿瘤演化中的重要性 ,为靶向于VEGF受体的基因治疗策略选择裸鼠动物模型提供了参考依据

Expression and Significance of VEGF Receptors in the Nude Mouse Models with Various Human Tumors

Objective To study the expression of vascular endothelial growth factor (VEGF) receptors flt\|1 and KDR in the nude mouse models with various human tumors subcutaneously transplanted by immunohistochemical method.Methods\ Tumor tissues were collected from the transgenic mice and washed,fixed,parraffined,sliced continuously and immunohistochemically stained.Results\ flt\|1 was determined to be strongly positive or positive in the vascular endothelial cells and tumor cells of 11 nude mouse models with various human tumors, that is,human gastric adenocarcinoma SGC,human malignant melanoma A375,human breast cancer Bcap\|37,human glioma U251,human intestinal adenocarcinoma LOVO,human hepatoma SMMC\|7721,human cervial cancer,human gastric adenocarcinoma SPC\|A1,human non\|small cell lung cancer H128,human ovarian cancer 3AO,human hepatoma graft,while KDR was determined to be positive or weakly positive in the vascular endothelial cells and tumor cells of 6 nude mouse models with various human tumors, that is,human gastric adenocarcinoma SGC,human maliganant melanoma A375,human breast cancer Bcap\|37,human glioma U251,human intestinal adenocarcinoma LOVO,human ovarian cancer 3AO.Conclusion\ Coexpression of VEGF receptors in tumor vascular endothelial cells and tumor cells raises the possibility of autocrine stimulation and emphasizes the importance of the reaction of VEGF and VEGF receptors to tumor growth.