人发角蛋白人工腱材料体内降解及生物相容性研究

目的研究人发角蛋白人工腱(humanhairkeratinartificaltendon,HHKAT)材料在体内的可降解性及其生物相容性.方法对12只日本大耳白兔随机分组,在脊旁肌埋藏不同处理时间的人发角蛋白人工腱试件F及Z,用正常人发O做对照,分别在2、6、12、24周取材,观察人发角蛋白的降解吸收过程及其周围的组织反应.结果动物植入实验中发现不同时间处理的材料其降解速度不同,其中降解最快的F组在24周已完全吸收,而Z组在24周只有部分降解,O组未见降解.HHKAT及人发在肌肉组织内无明显的炎症排斥反应,随着HHKAT材料的降解吸收,其周围的组织反应逐渐降低.结论本研究表明人发角蛋白人工腱材料具有良好的生物相容性,在体内能够被降解吸收,可根据不同的需要调节其降解速度,是良好的肌腱替代材料.

Degradation and Biocompatibility Investigation of Human - hair Keratin Artificial Tendon Material in Body

Objective To investigate the degradation of Human\|hair keratin artificial tendon (HHKAT) material and evaluate its biocompatibility in body. Methods The HHKAT materials were divided into two groups: Z and F according to disposed time, with normal human hair used as control group O. The Z, F and O samples were implanted into the two sides of spine muscle in 12 rabbits. The rabbits were killed 2,6,12,24 weeks after operation, observing the degradation progress of HHKAT and its tissue reaction. Result The degradation time of HHKAT was different according to disposed time: group F absorbed completely, group Z absorbed a little in 24weeks, while O groups showed no absorption. Histologic investigation showed that HHKAT and normal human hair did not evoke apparent inflammation reaction, and the histologic reaction decreased with degradation of HHKAT. Conclusion HHKAT is a kind of material with good biocompatibility, and can be in degradation according to requirement.