猴B病毒抗体ELISA检测方法的建立和应用研究

目的　建立猴B病毒抗体ELISA检测方法。方法　采用方阵滴定法 ,比较不同血清稀释度、3种酶结合物、2种抗原对检测结果的影响 ,与国外同类参比实验室进行检测结果的比较 ,确定ELISA法的最佳实验条件。结果　HSV抗原包被浓度 10 μg ml,抗人IgG HRP为 1∶2 0 0 0时 ;或B病毒抗原包被浓度 10 μg ml,抗人IgG HRP为1∶4 0 0 0时 ,阴性血清和阳性血清的A值差距最大。与国外B病毒检测专业实验室的检测结果符合率分别为98 0 2 %和 97 5 2 %。结论　建立了猴B病毒抗体的ELISA检测方法 ,提高了检测方法的准确性。

Establishment and Application of ELISA for Detection of Antibody to Simian B Virus

To provide a series studies for influence of the left lung inverse circulation and BCPA on pulmonary physiology and function and probably to use this technique to treat the patients with functional single ventricle with pulmonary hypertension, the reversal model of left pulmonary circulation (RLPC) with bidirectional cavopulmonary anastomosis (BCPA) was established in canines. Six mongrel dogs were chosen to be studied on this model. The procedure of establishing RLPC plus BCPA include anastomose the distal end of left pulmonary artery (LPA) to the left auricle; use a graft tube to connect the proximal LPA to three pulmonary veins and do a end to side anastomosis between supra vena cava and right pulmonary artery. All canines were survived over three days. Echo and angiography showed that there were no remarkable obstruction in left lung inverse circulation and cavopulmonary anastomosis. So the RLPC plus BCPA is technically feasible, it affords a experimental model for in vivo study of pulmonary pathophysiology and haemodynamics in complex congenital heart diseases kids with pulmonary hypertention and it''s a interm palliation and can be transit to total cavapulmonary shunts.