疏肝、健脾、补肾复方对慢性束缚应激大鼠行为学和免疫功能的影响

目的　研究慢性束缚应激时大鼠行为学和免疫功能的变化以及疏肝、健脾、补肾三种中药复方对其影响。方法　用特制束缚架连续束缚 2 1d(或 7d) ,每天 3h的方法制作大鼠束缚应激模型 ,观察大鼠行为学和免疫功能的变化。结果　慢性束缚应激后 ,大鼠的行为学发生了明显的变化 ,血清IL - 1β 上升 ,IL - 2和IL - 6下降 ,并且随着束缚时间的延长变化越明显。结论　逍遥散、四君子汤和金匮肾气丸能改善大鼠行为学的变化 ,明显增强慢性束缚应激大鼠的免疫功能。

The Effect of Compounds of Soothing Liver,Invigorating Spleen,Tonifying Kidney on the Praxiology and Immunological Function of Chronic Immobilization Stressed Rats

Nine random primers were used to amplify genomic DNA with randomly amplified polymorphic DNA (RAPD) methods in 3 inbred strains of rats. The result showed that the amplified band patterns of every individuals were same in one strain, the 3 strains of rats were homogeneous at these RAPD markers, however, the amplified band patterns showed different among three strains. The similarities between Wistar and WKY, Wistar and F344, WKY and F344 were 87.9%?98.5% and 89.2% respectively. The result indicated that the RAPD technique can be used as a method to monitor genetic contamination in inbred rats and to distinguish different strains of rats.