| 蝎毒多肽抑制白血病干细胞龛内活化的实验研究 |
| |
| 引用本文： | 杨向东,杨文华,史哲新,刘宝山,高宏,张蕾,陈艳鑫.蝎毒多肽抑制白血病干细胞龛内活化的实验研究[J].天津中医药大学学报,2011,28(4):329-331. |
| |
| 作者姓名： | 杨向东 杨文华 史哲新 刘宝山 高宏 张蕾 陈艳鑫 |
| |
| 作者单位： | 天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193;天津中医药大学第一附属医院血液科, 天津, 300193 |
| |
| 基金项目： | 天津市教委计划资助项目(OTJCYBJC1150) |
| |
| 摘 要： | 目的]观察蝎毒多肽(PESV)抑制白血病干细胞(LSC)在骨髓龛内的活化效应。方法]分离白血病/急性淋巴细胞白血病(AML/ALL)患者LSC,置于Transwell小室中,分为高中低剂量组及对照组,分别加入不同浓度蝎毒多肽后培养,计算不同浓度组及不同时间段的LSC迁移率。结果]PESV组迁移率分别为(25.01±6.83)%、(36.85±3.83)%、(50.73±7.15)%,与对照组(49.10±6.12)%比较差异具有显著性(P<0.05),高剂量组迁移率高于中低剂量组。结论]不同浓度PESV均有抑制白血病干细胞活化作用,抑制强度呈浓度依赖。
|
| 关 键 词： | 蝎毒多肽 白血病干细胞 龛 活化效应 |
| 收稿时间： | 2011/4/10 0:00:00 |
Experiment research on PESV for inhibiting activation of leucemia stem cell in the niche |
| |
| YANG Xiang-dong,YANG Wen-hu,SHI Zhe-xin.Experiment research on PESV for inhibiting activation of leucemia stem cell in the niche[J].Journal of Tianjin University of Traditonal Chinese Medicine,2011,28(4):329-331. |
| |
| Authors: | YANG Xiang-dong YANG Wen-hu SHI Zhe-xin |
| |
| Institution: | Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China;Department of Hematology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
| |
| Abstract: | Objective] To observe the effect of PESV(peptide extract from scorpion venom) for inhibiting the activation of leucemia stem cells(LSC) in the niche.Methods] LSC were isolated from patients with AML/ALL and stored in the Trans well.Then they were divided into four groups: high-dose group,middle-dose group,low-dose group and the positive control group.After cultured with different concentrations of PESV,the transport ratios of LSC in various concentration groups were computed at different time.Results] The transport ratios cultured with different concentrations of PESV were(25.01±6.83)%,(36.85±3.83)%,(50.73±7.15)%,and there was a significant difference compared with that of the positive control group(49.10±6.12)%(P<0.05).The transport ratio in the high-dose group is more than that of the middle and lose-dose groups.Conclusion] All different concentrations of PESV have the inhibiting effects against the activation of LSC with a inhibiting intensity depending on the concentrations of PESV. |
| |
| Keywords: | PESV LSC niche activating effect |
|
| 点击此处可从《天津中医药大学学报》浏览原始摘要信息 |
| 点击此处可从《天津中医药大学学报》下载免费的PDF全文 |
|
