丹酚酸B预处理EPCs联合BMSc移植对AMI大鼠心功能的影响

目的]通过建立大鼠急性心肌梗死模型,将中药丹酚酸B预处理的内皮祖细胞(EPCs)按不同比例与骨髓基质细胞(BMSc)进行混合后注射至缺血局部,观察细胞移植后大鼠急性心肌梗死模型心功能的改善情况。方法]应用密度梯度离心法和贴壁筛选法培养、纯化EPCs与BMSCs;采用结扎大鼠左冠状动脉前降支,制作大鼠急性心肌梗死模型。丹酚酸B最佳药物浓度处理的EPCs,以不同比例(1:1,2:1,4:1,8:1)与BMSc混合,在大鼠心肌梗死区周边分5点注射。结果]各细胞移植组均能不同程度的改善心肌梗死大鼠心室结构,增强心功能。超声心动图结果:同一时间细胞移植组均能不同程度的改善心肌梗死大鼠心室结构,增强心功能,与模型组相比,4:1组、8:1组左室射血分数及心输出量明显升高(P<0.05)。结论]丹酚酸B预处理的EPCs联合BMSc的移植促进了AMI大鼠左室功能的恢复。

Effects of EPCs preconditioned with salvianolic acid B combined with BMSc transplantation on heart function in rats with infracted myocardium

Objective] The acute myocardial infarction was established in rats.and then the rats were injected with endothelial progenitorcells (EPCs) preconditioned with Salvianolic acid B in different proportion combined with bone mesenchymal stem cells (BMSc) into theirheart ischemia area.The cardiac function of rats after cellular transplantation was observed.Methods] The mononuclear cells were separated from rat bone marrow by density-gradient centrifugation using lymphocyte isolation and then the cells were seeded on fibronectincoated culture flask and purified.The acute myocardial infarction (AMI) was produced in rats by ligation of the left coronary arterythrough left thoracostomy.Different proportion of endothelial progenitor cells preconditioned with Salvianolic acid B combined with bonemesenchymal stem cells (BMSc) was injected into the five points of heart ischemia area.Results] The structure of ventricles and cardiacfunction was showed enhanced in each cell transplanted groups.Compared with the control group, the left ventricular ejection fractionand the left ventricular cardiac output significantly increased in group 4:1 and 8:1 (P<0.05).Conclusion] The (EPCs) preconditionedwith Salvianolic acid B combined with bone mesenchymal stem cells (BMSc) may increase the transplanted cell of myocardial infarctionarea in rat, improve the functions of left ventricle.