| 不同单位保存的艾氏腹水癌细胞(EAC)的某些生物学特性比较研究 |
| |
| 引用本文： | 徐增年,王秀芳,吕占军,刘军须,刘福英.不同单位保存的艾氏腹水癌细胞(EAC)的某些生物学特性比较研究[J].实验动物与比较医学,2001(2):123-127. |
| |
| 作者姓名： | 徐增年 王秀芳 吕占军 刘军须 刘福英 |
| |
| 作者单位： | 徐增年（河北医科大学实验动物学部,石家庄 050017）
王秀芳（河北医科大学实验动物学部,石家庄 050017）
吕占军（河北医科大学实验动物学部,石家庄 050017）
刘军须（河北医科大学实验动物学部,石家庄 050017）
刘福英（河北医科大学实验动物学部,石家庄 050017） |
| |
| 基金项目： | 本研究为国家"九五”攻关课题(96-A23-06-03) |
| |
| 摘 要： | 目的比较研究不同单位保种的EAC瘤株生物学特性的差异.方法选择研究了北京市肿瘤研究所、山东省医学科学院药物研究所及武汉大学保种中心保存EAC细胞的大小,细胞株体外培养时对血清的依赖性,接种小鼠皮下建立肿瘤动物模型肿块生长曲线,建立的动物模型对环磷酰胺CTX治疗的敏感性等指标.结果发现三个单位保存的EAC细胞形态及大小基本一致;北京EAC体外培养容易生长,7.5%NCS即可生长良好,山东及武汉EAC体外培养生长力较弱;皮下接种BALB/c小鼠后,北京EAC最易生长肿瘤,武汉EAC次之,山东EAC最差;建立的动物模型对CTX治疗的敏感性,依次为北京EAC,武汉EAC,山东EAC,用CTX治疗的抑瘤率相应为82.9%,71.9%,50%.结果可见三个不同单位保种的EAC瘤细胞生物学特性确已存在显著差异.
|
| 关 键 词： | 癌 Ehrlich瘤 生物学鉴定法 动物/模型 |
| 修稿时间： | 2000/11/30 0:00:00 |
The Comparison Study on Biological Characteristics of EAC Cells from 3 Different Institutes |
| |
| XU Zengnian,WANG Xiufang,LU Zhanjun,LIU Junxu,LIU Fuying.The Comparison Study on Biological Characteristics of EAC Cells from 3 Different Institutes[J].Laboratory Animal and Comparative Medicine,2001(2):123-127. |
| |
| Authors: | XU Zengnian WANG Xiufang LU Zhanjun LIU Junxu LIU Fuying |
| |
| Abstract: | Objective\ To compare biological characteristics of EAC cells from 3 different institutes.Methods\ Cell size was determined with the Cells Analysis System.The curve changes of neoplastic diameters were studied after subcutaneous injection of EAC in BALB/c.The neoplastic weights of the 12th day were measured and the sensitivity of EAC to CTX in BALB/c was compared.Results\ Both size and shape of cells were basicly of similarity.Ectogenesis of Beijing EAC was easier than the other two EAC. Induced tumors of Beijing EAC were the most by subcutaneous inoculation. The tumor-inhibitory rate to CTX treatment was 82.9%(Beijing EAC),71.9%(Wuhan EAC),50%(Shandong EAC) respectively. Conclusion\ The EAC cells from 3 different institutes appeared to be different markedly. |
| |
| Keywords: | Carcinoma Ehrlich tumor Biological assay Animals/models |
|
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
| 点击此处可从《实验动物与比较医学》下载免费的PDF全文 |
|
