慢性乙型肝炎病毒感染产妇的胎儿脐带血树突状细胞的表型及功能

目的探讨孕妇慢性乙型肝炎病毒(HBV)感染对其胎儿脐血的树突状细胞(DC)的表型和功能的影响,并与健康胎儿、健康成人及成人慢性HBV感染者进行对比研究。方法体外分离慢性HBV 感染产妇及健康产妇胎儿脐带血、健康成人及慢性乙型肝炎患者外周血单个核细胞进行树突状细胞转化,行流式细胞术对其表型进行分析,通过交叉混合淋巴细胞培养和DC培养上清液白细胞介素(IL)-12检测来分析DC的功能。结果慢性HBV感染产妇脐血来源DC的CD 80、CD 83的表达显著低于健康胎儿组、健康成人组、成人慢性HBV感染组,CD14的表达则显著高于此三组,P值均<0.0 5;慢性HBV感染产妇脐带血DC分泌的IL-12水平也明显低于健康产妇、健康成人组及慢性乙型肝炎患者(P<0.05);促异体T淋巴细胞增殖能力的强弱依次为健康成人DC-健康脐血T淋巴细胞>健康成人DC-健康成人异体T淋巴细胞>健康脐血DC-异体健康脐血T淋巴细胞>健康脐血DC-健康成人T淋巴细胞>孕母HBV感染脐血DC-健康脐血T淋巴细胞>孕母HBV感染脐血DC-健康异体成人T淋巴细胞。结论慢性HBV感染产妇胎儿脐血DC的成熟和功能显著低于健康胎儿脐血DC、健康成人甚至慢性乙型肝炎患者外周血DC。

Study of phenotypes and functions of cord blood dendritic cells from fetuses whose mothers had chronic hepatitis B

OBJECTIVE: To investigate the phenotypes and functions of cord blood dendritic cells of fetuses whose mothers are patients with chronic hepatitis B. METHODS: Peripheral blood and cord blood mononuclear cells (PBMC) were isolated from whole blood by density gradient centrifugation with Ficoll-Hypaque. The adherent cells were cultured in AIM-V medium containing recombinant human IL-4, TNF-alpha and GM-CSF. On day 9, mature DCs (mDC) were harvested and used for phenotype analysis. The amounts of IL-12 which dendritic cells produced were measured. The dendritic cells that were studied and compared were from cord blood of fetuses of both CHB positive and negative mothers and from CHC adult peripheral blood. RESULTS: The expression rate of CD80 and CD83 of chronic hepatitis B mother cord blood dendritic cells was low compared with that of the healthy cord blood, healthy adult peripheral blood, and chronic hepatitis B adult peripheral blood, P < 0.05. The amount of IL-12 produced by chronic hepatitis B mother cord blood dendritic cells was lower than that of healthy cord blood, healthy adult peripheral blood, chronic hepatitis B adult peripheral blood (P < 0.05). The T lymphocyte proliferation inducing ability of dendritic cells of healthy adult peripheral blood was higher in inducing cord blood T lymphocytes proliferation, which was greater than that of the healthy adult peripheral blood in inducing adult T lymphocytes and was greater than that of the healthy cord blood dendritic cells in inducing cord blood T lymphocytes, which was greater than that of the healthy cord blood in inducing adult T lymphocytes, which was greater than that of chronic hepatitis B mothers in inducing cord blood T lymphocytes, which was greater than that of chronic hepatitis B mother cord blood in inducing adult T lymphocytes. CONCLUSION: The maturation and functioning of CHB mother cord blood dendritic cells were lower than those of healthy cord blood, healthy adult peripheral blood and CHB adult peripheral blood.