凝集素受体在叶酸,维生素C拮抗环磷酰胺致神经管畸形中的作用研究

为探讨凝集素受体在叶酸，维生素Ｃ防止歪磷酰酰胺致大鼠胚胎神经管畸形中的作用。方法：实验组和对照组孕鼠均于给环磷酰胺后２４小时剖腹取胎，常规石蜡包埋切片，用刀豆凝集素，花生凝集素和荆豆凝集素为分子探针作亲合组织杂色。对两组胚胎神经管的神经上皮，界膜及神经管周围间充质相应糖基进行定性，定位和半定一的比较观察。

THE ROLE OF LECTIN RECEPTORS IN FOLIC ACID AND VITAMIN C WHICH PREVENTS NEURAL TUBE DEFECTS INDUCED BY CYCLOPHOSPHAMIDE IN RATS

In order to explore the roles of lectin receptors in folic acid and vitamin C which prevents neural tube defects(NTD) induced by cyclophosphamide(CP). pregnant rets in the treated group (folic acid and vitramin C) and the control group were dissected at 24 hours after being given CP. The embryos were routinely embedded with paraffin and sectioned in series. The ConA. PNA. and UEA were used as molecule probes to explore the related oligosaccharides-lectin receptors of the neural epithelium, the limiting membrane and the mesenchyme around the neural tube and a comparative observation was made on their quality, distribution and semiquantity. The results showed great stain intensity for ConA and PNA in the control group, suggesting that CP could increase the quantity of the receptor glycoproteins in the neural epithelium; thus interfering with the normal process of cell proliferation, migration, adhesion and recognition, and causing neural tube defects. Stainings in the treated group was significantly weaker than that in the control group, suggesting that the folic acid and vitamin C could inhibit the abnormal increase of the related receptor glycoproteins. and protect the normal process of cell proliferation and imigration from the interference of CP. which might be another important way for folic acid and vitramin C to prevent neural tube defects.