Frequencies of glutathione s-transferase (GSTM1, GSTM3 AND GSTT1) polymorphisms in a Malaysian population |
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Authors: | Mustafa A. Alshagga Norazlina Mohamed Ahmad Nazrun Suhid Ibrahim Abdel Aziz Ibrahim Syed Zulkifli Syed Zakaria |
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Affiliation: | 1.Department of Pharmacology, Faculty of Medicine, Cyberjaya University College of Medical Sciences (CUCMS), Selangor, Malaysia;2.Department of Pharmacology, UKM Medical Centre, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;3.Universiti Kebangsaan Malaysia Medical Molecular Biology Institute (UMBI), UKM Medical Centre, Universiti Kebangsaan Malaysia (UKM), Malaysia |
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Abstract: | IntroductionGlutathione S-transferase (GST) is a xenobiotic metabolising enzyme (XME), which may modify susceptibility in certain ethnic groups, showing ethnic dependent polymorphism. The aim of this study was to determine GSTM1, GSTM3 and GSTT1 gene polymorphisms in a Malaysian population in Kuala Lumpur.Material and methodsBlood or buccal swab samples were collected from 137 Form II students from three schools in Wilayah Persekutuan Kuala Lumpur. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).ResultsGlutathione-S-transferase GSTM3 gene frequencies were 89% for AA, 10% for AB and 1% for BB. The gene frequencies for deleted GSTM1 and GSTT1 were 66% and 18% respectively.ConclusionsThis study suggested that the Malay population is at risk for environmental diseases and provides the basis for gene-environment association studies to be carried out. |
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Keywords: | gene glutathione S-transferase polymorphism Malaysians |
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