寄生于黄牛与水牛的肉孢子虫同工酶研究

目的 比较几种肉孢子虫过氧化物酶、磷酸葡萄糖异构酶同工酶酶谱。 方法 将肉孢子虫包囊从黄牛和水牛肉中剖离，经超声粉碎、高速离心后取上清，聚丙烯酰胺垂直平板梯度凝胶电泳染色进行同工酶酶谱分析。 结果 黄牛和水牛的枯氏肉孢子虫两种酶酶带相似，其中过氧化物酶都出现7条带，位于pH6.98~4.41之间，磷酸葡萄糖异构酶出现6条带，pH位于6.53~4.66之间；黄牛和水牛的毛状肉孢子虫的两种酶酶带相似，其中过氧化物酶都出现5条带，pH位于7.15~4.97之间，磷酸葡萄糖异构酶出现4条带，pH位于6.51~4.70之间。枯氏肉孢子虫、毛状肉孢子虫、梭状肉孢子虫在同工酶酶谱上存在明显的差异，酶带数目、酶带的pH值分布均不同。 结论 同工酶谱分析证明黄牛和水牛都是枯氏肉孢子虫、毛状肉孢子虫自然中间宿主。枯氏肉孢子虫、毛状肉孢子、梭状肉孢子虫为不同的虫种。

A Comparative Study on Isoenzymes of Sarcocystis spp. fromCattle and Water Buffaloes

Objective To analyze the zymogram of peroxidase(PER)and phosphoglucose isomerase(PGI)of three species of Sarcocystis.Methods The collected parasites were homogenized and fragmented by ultrasonication.After centrifugation,the supernatants were analyzed by isoelectric focusing electrophoresis.Results The isolates of S.cruzi from infected water buffalo and cattle all showed identical enzyme profiles,7 bands of PER at pH4.44~6.98 and 6 bands of PGI at pH 4.66~6.53;and same with the isolates of S.hirsuta.5 bands of PER at pH 4.97~7.15 and 4 bands of PGI at pH 4.70~6.51.The zymograms among S.cruzi,S.hirsuta and S.fusiformis were different considerably.Conclusion The data support the hypothesis that both water buffalo and cattle are the natural intermediate hosts of S.cruzi and S.hirsuta at the gene level.S.cruzi,S.hirsuta and S.fusiformis are different species.