2型糖尿病心脑血管病变与血小板活化、内皮损伤的临床研究

观察 5 0例 2型糖尿病患者及 2 0例正常者 ,根据是否伴心脑血管病变 ,将糖尿病患者组分为两个亚组。应用流式细胞术 ( FCM)检测血小板膜蛋白 CD62 p、CD63的表达 ,并联合观察各组血清 ET- 1浓度及相关指标。结果发现 ,血小板膜蛋白 CD62 p、CD63的阳性表达率单纯糖尿病组及糖尿病伴心脑血管病变组均高于正常对照组 ( P<0 .0 5 ) ,且糖尿病伴心脑血管病变组较不伴心脑血管病变组高 ( P<0 .0 5 ) ;血 ET- 1浓度糖尿病伴心脑血管病变组高于单纯糖尿病组 ( P<0 .0 5 )及正常对照组 ( P<0 .0 0 1) ;CD62 p、CD63二者高度相关 ( r=0 .811,P<0 .0 1)。提示血小板活化、内皮损伤参与糖尿病血管病变的发生机理 ,糖尿病糖与脂质代谢紊乱与此关系密切。CD62 p、CD63间高度相关 ,与 ET- 1都可作为 2型糖尿病伴心脑血管病变早期诊断和病情分析的理想指标

Relationship between diabetic vascular disease and platelet activation and endothelium injury

cases with diabetes mellitus(including 30 ones with cardiac or cerebral vascular disease and 20 cases without diabetic vascular complications)and 20 normal persons were observed The expression of the membrane proteins CD62p and CD63 were measured by whole blood flow cytometry(FCM) And we detected the plasma concentration of ET 1 was detected by radioimmunoassay Result showed that the positive expression of CD62p,CD63 and the plasma concentration of ET 1 were higher in the group of patients with diabetic cardiocerebral vascular disease than the other two groups(P<0 05) And they were also higher in the group of patients without diabetic cardiocerebral vascular disease than the normla contral(P<0 05) The findings susgest that platelet activation and endothlium injury contribute to the diabetic vascular diseases,which is associated to the disturbance of glucose and lipid closely With the application of the FCM,CD62p,CD63 are good indexes to early diagnosing and monitoring cardiocerebral vasular disease in diabetes patients as well as ET 1