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3,4-二-O-甲基-1,2,5,6-四硝酸甘露醇酯对冠状动脉结扎大鼠心肌缺血的保护作用
引用本文:詹天荣,贾思佳,袁华茂,宋金明,侯万国. 3,4-二-O-甲基-1,2,5,6-四硝酸甘露醇酯对冠状动脉结扎大鼠心肌缺血的保护作用[J]. 中国药学杂志, 2012, 47(3): 190-193
作者姓名:詹天荣  贾思佳  袁华茂  宋金明  侯万国
作者单位:中国科学院海洋研究所;青岛科技大学化学与分子工程学院国家生态化工重点实验室培育基地教育部重点实验室胶体与界面实验室
基金项目:中国科学院知识创新工程重要方向项目(KZCX2-YW-209);山东省泰山学者基金(ts20070713);山东省自然科学基金(ZR2009BM022);山东省博士后创新项目专项资金(200902027);国家海洋局海洋生态环境科学与工程重点实验室开放基金(MESE-2010-08)资助
摘    要: 目的 研究3,4-二-O-甲基-1,2,5,6-四硝酸甘露醇酯(3,4-di-O-methyl-1,2,5,6-D-mannitol tetranitrate,DMMTN)对冠状动脉结扎所致大鼠急性心肌缺血的保护作用及其作用机制。方法 以结扎左冠状动脉前降支方法建立大鼠急性心肌缺血模型,建立假手术组、心肌梗死模型组、鲁南欣康组、DMMTN低、中、高剂量(15,30,60 mg·kg-1)组,测定手术后不同时段心电图ST段和T段的抬高;并于手术3 h后硝基四氮唑蓝( NBT) 染色法测定心肌梗死面积;测定血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)和超氧化物歧化酶(SOD)。结果 DMMTN能显著缩小心肌梗死面积,抑制术后心电图ST段和T段的抬高;能显著降低CK和LDH的活力,以及MDA的含量,同时能显著提高SOD的活力。结论 DMMTN对冠状动脉结扎所致大鼠急性心肌缺血具有显著的保护作用。

关 键 词:3  4-二-O-甲基-1  2  5  6-四硝酸甘露醇酯|冠状动脉结扎|心肌缺血|大鼠
收稿时间:2012-03-01;

Protective Effects of 3,4-di-O-methyl-1,2,5,6-D-Mannitol Tetranitrate on Myocardial Ischemia Induced by Ligating Coronary Artery in Rats
ZHAN Tian-rong,JIA Si-jia,YUAN Hua-mao,SONG Jin-ming,HOU Wan-guo. Protective Effects of 3,4-di-O-methyl-1,2,5,6-D-Mannitol Tetranitrate on Myocardial Ischemia Induced by Ligating Coronary Artery in Rats[J]. Chinese Pharmaceutical Journal, 2012, 47(3): 190-193
Authors:ZHAN Tian-rong  JIA Si-jia  YUAN Hua-mao  SONG Jin-ming  HOU Wan-guo
Affiliation:1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;2.State Key Laboratory Base of Eco-chemical Engineering,Lab of Colloids and Interfaces,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China)
Abstract:OBJECTIVE To study the effects of 3,4-di-O-methyl-1,2,5,6-D-mannitol tetranitrate(DMMTN) on acute myocardial ischemia induced by ligating coronary artery in rats.METHODS Acute myocardial ischemia model was developed by ligating the anterior descending branch of left coronary artery in rats.The rats were randomly divided into 6 groups: sham group,model group,Lunanxinkang group,low-dose(15 mg·kg-1) DMMTN group,middle-dose(30 mg·kg-1) DMMTN group and high-dose(60 mg·kg-1) DMMTN group.The changes of ST segment and T segment of electrocardiogram at different moment were recorded after myocardial ischemia injury.At 3 h after ligation,the myocardial infarct size was measured by nitroblue tetrazolium(NBT) staining method.And the activities of creatine phosphokinase(CK),lactate dehydrogenase(LDH) and superoxide dismutase(SOD),and the content of malondialdehyde(MDA) were determined to evaluate the protective effects of DMMTN.RESULTS DMMTN could reduce the size of myocardial infarct significantly,inhibit the rising of ST segment and T segment of electrocardiogram,decrease the level of CK,LDH and MDA,and increase the activity of SOD.CONCLUSION DMMTN showed obvious protective effects on acute myocardial ischemia induced by ligating the coronary artery in rats.
Keywords:3,4-di-O-methyl-1,2,5,6-D-mannitol tetranitrate  ligating coronary artery  myocardial ischemia  rat
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