| 应用巢式PCR对恶性疟原虫培养中支原体污染的检测 |
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| 引用本文： | 胡东伟,丁帅,胡守锋,孙新. 应用巢式PCR对恶性疟原虫培养中支原体污染的检测[J]. 中国病原生物学杂志, 2012, 0(1): 32-33,29 |
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| 作者姓名： | 胡东伟 丁帅 胡守锋 孙新 |
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| 作者单位： | 蚌埠医学院安徽省感染与免疫重点实验室;第二军医大学病原生物学教研室 |
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| 摘 要： | 目的应用巢式PCR检测恶性疟原虫体外培养中的支原体污染。方法根据支原体16s和23s保守序列设计PCR引物,应用巢式PCR检测恶性疟原虫体外培养中的支原体。结果体外培养的恶性疟原虫标本16份,PCR检测支原体均阳性,经测序比对后确认为口腔支原体。已知阴性对照无扩增带。结论应用巢式PCR能敏感和特异地检出恶性疟原虫体外培养中的支原体。
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| 关 键 词： | 支原体 疟原虫,恶性 培养 巢式PCR |
Use of nested PCR to detect Mycoplasma contamination in vitro in cultured Plasmodium falciparum |
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| HU Dong-wei,DING Shuai,HU Shou-feng,SUN Xin. Use of nested PCR to detect Mycoplasma contamination in vitro in cultured Plasmodium falciparum[J]. Journal of Pathogen Biology, 2012, 0(1): 32-33,29 |
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| Authors: | HU Dong-wei DING Shuai HU Shou-feng SUN Xin |
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| Affiliation: | 1(1.Bengbu Medical College,Anhui Provincial Key Laboratory of Infection and Immunity,Bengbu 233030,Anhui,China;2.Department of Pathogen Biology of The Second Military Medical University,200438 Shanghai,China) |
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| Abstract: | Objective To detect Mycoplasma contaminants in vitro in cultured Plasmodium falciparum and then to verify the contaminating species.Methods Culture isolates of Plasmodium falciparum were screened for the presence of Mycoplasma using nested PCR with two sets of primers,and the16S-23S rRNA sequence was amplified.Results All 16 isolates were clearly determined to be positive for Mycoplasma according to nested PCR.The sequence of 16S-23S rRNA was compared to the reference sequence for Mycoplasma species.Contaminants in the culture were identified as Mycoplasma orale.Conclusion The results of a nested PCR assay proved to be a sensitive and specific indicator of Mycoplasma contamination in vitro in culturedPlasmodium falciparum. |
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| Keywords: | Mycoplasma Plasmodium falciparum culture nested PCR |
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