益气强心饮对慢性心衰大鼠心肌细胞凋亡及Bax、Bcl-2基因蛋白表达影响的实验研究

[目的]本实验旨在通过动物实验观测中药益气强心饮对慢性心衰大鼠心肌细胞凋亡及Bax、Bcl-2基因蛋白表达的影响。[方法]采用腹主动脉缩窄法结合皮质激素造模法建立慢性心衰阳虚水泛证大鼠模型,随机分为模型组、西药对照组、中药对照组、中药低剂量组、中药中剂量组、中药高剂量组、假手术组,用药8周后,观察各组大鼠的心肌细胞凋亡及Bax、Bcl-2基因蛋白表达。[结果]益气强心饮能明显下调慢性心衰大鼠心肌组织Bax基因蛋白表达、上调Bcl-2基因蛋白表达,疗效等同西药对照组。[结论]益气强心饮可调控慢性心衰大鼠的心肌细胞凋亡,具有治疗及延缓心衰发展等作用。

Yiqi Qiangxin Drink Chronic Heart Failure on Myocardial Apoptosis and Bax, Bcl-2 Gene Expression in Rats

[ Objective] This study was designed by observing YIqi Qiangxin Drink animals with chronic heart failure on myocardial apoptosis and Bax, Bcl -2 protein expression. [ Method] abdominal aortic coarctation combined with corticosteroids in chronic heart failure was established by modeling the water pan card yang deficiency rats were randomly divided into model group, comparison group, control group of Chinese medicine, Chinese medicine low dose group, Chinese herbal medicine dose group, Chinese high -dose group, sham operation group, After 8 weeks of treatment, rats of myocardial apoptosis and Bax, Bcl - 2 gene expression. [ Results] YIqi Qiangxin Drink significantly reduced myocardial tissue of rats with chronic heart failure Bax protein expression, regulated Bcl -2 protein expression, effect the same comparison group, [Conclusion] YIqi Qiangxin Drink chronic heart failure can be regulated cardiomyocyte apoptosis in rats with heart failure treatment and delay the development of the role.