重组骨形态发生蛋白—7基因转染软骨细胞的实验研究

目的：研究外源性人重组骨形态发生蛋白-7(rhBMP-7)基因在转染的软骨细胞中的表达情况。方法：用脂质体介导法将rhBMP-7转入兔关节软骨细胞，分别用免疫组化染色和逆转录－聚合酶链式反应（RT－PCR）检测其表达，同时用四甲基偶氮唑蓝微量酶反应比色法（MTT法）检测了表达产物的活性。结果：48h后转染的软骨细胞有明显的pcDNA3-rhBMP7基因表达，转染细胞的表达产物对正常软骨细胞的增殖有显著的促进作用。结论：外源性rhBMP-7基因可在软骨细胞中获得高效表达，这为关节疾病的基因治疗提供了理论基础。

Study of Chondrocytes Transfected by Bone Morphogenetic Protein-7 Gene

Objective:To investigate the exogenous bone morphogenetic protein-7 gene expression in transfected chondrocytes.Methods:With a liopfectamine vector,the plasmid pcDNA 3-rhBMP7 was transferred to rabbit articular chondrocytes that were cultured in vitro,and chondrocytes which transduced with the plasmid pcDNA 3 severed as the control.The transient expression were tested by immunohistochemical staining and RT-PCR assay after the transfection.The effected of gene transfer on the proliferation were detected by MTT.Results:The rhBMP-7 gene expression could be detected in the tansfected chondrocytes after 48 hours.And the tansfected chondrocytes could stimulate the proliferation of chondrocytes.dConclusions:The bone morphogenetic protein-7 could be expressed effectively in transfected chondrocytes by pcDNA 3-rhBMP7 plasmid.This laid foundation of the gene therapy of articular disease.