| HPLC法同时测定夏天无中原阿片碱和延胡索乙素 |
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| 引用本文： | 胡佳,张振秋,齐建波. HPLC法同时测定夏天无中原阿片碱和延胡索乙素[J]. 中华中医药学刊, 2006, 24(7): 1355-1356 |
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| 作者姓名： | 胡佳 张振秋 齐建波 |
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| 作者单位： | 辽宁中医药大学药学院,辽宁,沈阳,110032 |
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| 摘 要： | 目的:建立夏天无中原阿片碱和延胡索乙素的含量测定方法,为夏天无质量标准的研究提供科学依据。方法:采用高效液相色谱法同时测定夏天无中原阿片碱和延胡索乙素的含量。色谱条件为D iamonsil C18(5μm,150mm×4.6mm)柱,以甲醇-磷酸盐缓冲液(65:35)为流动相;检测波长为285nm。结果:此方法线性良好,平均加样回收率分别为99.40%,100.9%。RSD分别为2.2%,2.7%。结论:本方法精密度高,分离度良好,可用于同时测定夏天无中原阿片碱和延胡索乙素的含量。
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| 关 键 词： | 高效液相色谱法 夏天无 原阿片碱 延胡索乙素 |
| 文章编号： | 1009-5276(2006)07-1355-02 |
| 修稿时间： | 2006-01-12 |
Determination of Protopine and Tetrahydropalmatine in Rhizoma Decumbentis by HPLC |
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| Hu Jia,Zhang Zhenqiu,Qi Jianbo. Determination of Protopine and Tetrahydropalmatine in Rhizoma Decumbentis by HPLC[J]. Chinese Archives of Traditional Chinese Medicine, 2006, 24(7): 1355-1356 |
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| Authors: | Hu Jia Zhang Zhenqiu Qi Jianbo |
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| Abstract: | Objective:To establish the method for determining the content of protopine and tetrahydropalmatine in Rhizoma decumbentis by HPLC.Method:Using ODS-C18 column,methanol-buffer phosphate(65:35),detection wavelength at 285nm.Results:The average recovery was 99.40% and 100.9%.and relared standard deviation(RSD) was 2.2% and 2.7%.Conclution:This method can be used to determine the content of protopine and tetrahydropalmatine in Rhizoma decumbentis. |
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| Keywords: | HPLC Rhizoma decumbentis protopine tetrahydropalmatine |
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