百色少数民族地区结核病患者TTV和HGV感染的调查

目的了解百色少数民族地区结核病患者输血传播病毒(transfusion transmitted virus,TTV)和庚型肝炎病毒(hepeatitis G virus,HGV)的感染状况及临床意义。方法应用酶联免疫法(ELISA)检测712例结核病患者及500人健康人群血清抗-TTV和抗-HGV,对抗-TTV阳性者用PCR法检测TTV DNA,抗-HGV阳性者用逆转录套式聚合酶链反应法(PT-nPCR)检测HGV RNA,分析结核病患者TTV DNA或HGV RNA阳性者临床特征。结果结核病患者TTV感染率16.71%,健康人群5.60%;结核病患者HGV感染率14.61%,健康人群2.60%;配对比较差异有显著性(P<0.01);结核病患者TTV与HGV感染率具有随年龄增长呈递增趋势,男性高于女性(P<0.05),感染者有过输血史和注射史的比率比非感染者高(P<0.05),在抗结核治疗中感染者比非感染者容易出现抗结核药物所致肝损害(P<0.01)。结论结核病患者对TTV和HGV有较高的感染率,反复注射及免疫功能低下可能是增加TTV和HGV感染的原因,感染者在抗结核治疗中可能更容易出现肝损害。

Investigation for the infection of transfusion transmitted virus and hepatitis G virus in the patients with tuberculosis in Baise city

Objective To determine the infection status and clinical significance of transfusion transmitted virus(TTV) and hepatitis G virus(HGV) in the patients with tuberculosis in Baise minority nationality regions.Method Anti-TTV and anti-HGV were detected with enzyme linked immune absorption assay(ELISA) in the serums of 712 tuberculosis patients and 500 control subjects.TTV-DNA was detected with polymerase chain reaction(PCR) in the subjects which anti-TTV was positive.HGV-RNA was detected with PT-nPCR in the subjects which anti-HGV was positive.Clinical character of tuberculosis patients with positive TTV DNA or positive HGV-RNA was investigated.Results The infection rate of TTV was 16.71% in the tuberculosis patients and 5.60% in the control subjects.The infection rate of HGV was 14.61% in the tuberculosis patients and 2.60% the in control subjects.There is significant difference in two groups(P<0.01).The infection rate of TTV and HGV has a trend to rise by age in the tuberculosis patients.The rate in male group is higher than in female group(P<0.05).And the infected people have a higher rate of having blood transfusion and injection history than the uninfected people.Liver injury resulting from antitubercular drugs is more common occurred in the patients infected with TTV-DNA and HGV-RNA during tuberculosis therapy(P<0.01).Conclusion The results of the study suggest that the infection rate of TTV and HGV is in a relatively high level in the tuberculosis patients.Immune function damage and multi-injection may contribute to it.And the infected patients are easier to suffer the liver damage during tuberculosis therapy.