去甲二氢愈创木酸对胶质瘤细胞诱导的内皮细胞迁移的影响

目的 观察去甲二氢愈创木酸（ＮＤＧＡ）对胶质瘤细胞诱导的内皮细胞迁移的影响。方法 采用微孔滤膜培养小室及室细胞联合培养法,进行人脐静脉内皮细胞系ＥＣＶ－３０４细胞与人恶性胶质瘤细胞系ＳＨＧ－４４细胞的联合培养,并以免疫组化ＳＰ方法检测ＳＨＧ－４４细胞血管内皮生长因子（ＶＥＧＦ）、碱性成纤维细胞生长因子（ｂＦＧＦ）的表达。结果 １００μｍｏｌ／Ｌ的ＮＤＧＡ作用１～３ｄ后,ＳＨＧ－４４细胞ＶＥＧＦ、ｂ

The effect of nordihydroguaiaretic acid on endothelial cell migration induced by glioma cells

Objective　To investigate the effect of nordihydroguaiaretic acid (NDGA) on the migration of endothelial cells (ECs) induced by glioma cells (GCs) in vitro. Methods　Cells of human umbilical vein endothelial cell line ECV-304 and human malignant glioma cell line SHG-44 were co-cultured in the Falcon Cell Culture Insert system, and the effect of NDGA on the migration of ECs induced by GCs was investigated. The expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) was examined with immunohistochemistry. Results　The expressions of VEGF and bFGF in SHG-44 cells were reduced after treatment with 100 μmol/L NDGA for 1 to 3 days. 100 μmol/L NDGA significantly inhibited not only the chemotactic migration of ECs induced by either glioma cells or glioma-conditioned media, but also the random motility of ECs (P<0.01). NDGA at a concentration between 50 μmol/L and 200 μmol/L inhibited the chemotactic migration of ECs in a dose-dependent manner (the inhibition ratios were 12.0%,37.4%, 86.7% and 82.2%). Conclusion　The results confirm the inhibitory effects of NDGA on the migration and angiogenic factor expression of ECs, which suggests that NDGA may suppress angiogenesis of glioma.