CD40配体对套细胞淋巴瘤细胞系增殖的影响

套细胞淋巴瘤（MCL）是一种表达CD5的非霍奇金B细胞淋巴瘤,以中等大小B细胞侵入外套层为特征。CD40L与CD40间的相互作用对B细胞的增殖与分化有重要意义。对于不同阶段的B细胞,CD40L的刺激可产生抑制或诱导分化的作用。在本次研究中,利用SP49和SP53这两个套细胞淋巴瘤细胞系对CD40L反应作了研究。与表达CD40L的鼠淋巴细胞混合培养后,BrdU结合的SP49及SP53细胞减少了1/2～1/3,而BrdU结合的对照细胞系（包括Ramos,BJAB and BALL-1）无反应或增加。抗CD40L抗体在0～20 ng/ml时能封闭CD40L对SP49细胞增殖的抑制作用。这些结果提示CD40L在活体内可能对MCL增殖有抑制作用。

THE PROLIFERATION EFFECT OF CD40 LIGAND ON MANTLE CELL LYMPHOMA LINES

Mantle cell lymphoma （MCL） is a CD5 ＋ non- Hodgkin＇ s B -cell lymphoma characterized by the infiltration of intermediate sized B -cells into the mantle zones. Interaction between CD40L and CD40 is important for B cell proliferation and differentiation. CD40L stimulation can induce to their differentiation state. Previous re- ports examining the effect of stimulation via the CD40 cascade on ex vivo MCL cells have provided conflicting resuits. In this study, two MCL lines, SP49 and sp53, were examined for response to CD40L. Cocultivation with CD40L - expressing mouse L cells reduced the BrdU incorporation of SP49 and SP53cells by half to one - third, while BrdU incorporation of control cell lines, including Ramos, B JAB and BALL - 1, was not affected or increased. Anti - CD40L antibody blocked the CD40L inhibition of SP49 cell proliferation in dose - dependent manner in the range from 0 to 20ng/ml. These results suggested the possibility that CD40L may also inhibit MCL proliferation in vivo.