Synthesis,Properties, and Intratumoral Evaluation of Mitoxantrone-loaded Casein Microspheres in Lewis Lung Carcinoma |
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Authors: | W. A. Knepp A. Jayakrishnan J. M. Quigg H. S. Sitren J. J. Bagnall E. P. Goldberg |
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Abstract: | Abstract— Smooth, round, uniform bovine casein microspheres of 1–5 and 10–20 μm size were readily prepared by a steric stabilization technique previously developed in this laboratory for synthesis of albumin microspheres. The avid phagocytic uptake of casein and albumin microspheres was demonstrated with fluorescein-labelled microspheres using a macrophage-like mouse myelomonocytic leukaemia cell line. Post-synthesis loading of 25% mitoxantrone was achieved for casein microspheres containing 20% polyglutamic acid. Preliminary intratumoural chemotherapy experiments with a mouse Lewis lung carcinoma indicated that mitoxantrone and mitoxantrone-loaded casein-polyglutamic acid microspheres exhibited lower toxicity when administered intratumorally. |
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