p16蛋白及其基因在原发性肝细胞癌中的表达缺失性研究

目的通过p16蛋白及其基因在原发性肝细胞癌中的表达缺失研究,探讨p16基因与肝癌的关系.方法收集我院原发性肝癌32例,用免疫组织化学SP法、聚合酶链式反应(PCR)方法分别检测肝癌及癌旁组织中p16蛋白的表达及其基因的缺失情况.结果32例肝癌中,p16蛋白阴性表达率为62.5%(20/32),癌旁组织中p16蛋白阴性表达率为3.13%(1/32),两者差异有显著意义(P＜0.01).p16蛋白的表达与肝癌分化程度关系密切(P＜0.05),而与癌灶体积、甲胎球蛋白(AFP)值的差异无显著意义(P＞0.05).p16基因在癌组织中的缺失率25%(8/32),在癌旁组织为3.13%(1/32),相差显著(P＜0.05).p16基因缺失与肝癌分化程度、癌灶体积、AFP值无关(P＞0.05).结论p16基因缺失与肝癌发生关系密切,提示p16蛋白可能是预测病人预后的一个指标,但仍需进一步确证.

Study on p16 protein expression and p16 gene deletion in primary hepatocellular carcinoma

Objective: To study the p16 protein expression and p16 gene deletion in hepatocellular carcinoma (HCC), and to observe the relationship between p16 gene and HCC. Methods: Using immunohistochemistry and polymerase chain reaction (PCR), The expression of p16 protein and the deletion of p16 gene in 32 cases of HCC were examined. Results: The loss of p16 protein expression was observed in 20 cases of HCC (62.5%, 20/32) and in 1 case of tumor adjacent tissues (3.13%, 1/32), respectively (P<0.01). The loss of p16 protein expression was closely correlated with poor histological differentiation of the tumor (P<0.05), but not related to the size of the tumor and the serum AFP level (P>0.05). The deletion rate of p16 gene in HCC (25%, 8/32) was significantly different from that in tumor adjacent hepatic tissues (3.13%, 1/32). The deletion rate of p16 gene was not correlated with the histological differentiation, the size of the tumor and the serum AFP level (P>0.05). Conclusions: There is a close relationship between hepatocarcinogenesis and p16 gene inactivation. The expression of p16 protein may be an useful index to predict the prognosis.