| NT-3基因修饰施万细胞促进神经干细胞体外分化为神经元样细胞 |
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| 引用本文： | 郭家松,曾园山,李海标,黄文林,刘然义. NT-3基因修饰施万细胞促进神经干细胞体外分化为神经元样细胞[J]. 中国病理生理杂志, 2005, 21(1): 26-30. DOI: 1000-4718 |
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| 作者姓名： | 郭家松 曾园山 李海标 黄文林 刘然义 |
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| 作者单位： | 中山大学1中山医学院组织胚胎学教研室神经科学研究室，2肿瘤防治中心, 广东 广州 510080；3第一军医大学分校, 广东 广州 510315 |
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| 基金项目： | 国家重点基础发展规划资助项目(No.19990540009)，国家自然科学基金资助项目(No.30270700)，广东省社会发展攻关基金(2003C33808) |
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| 摘 要： | 目的：观测神经营养素-3（NT-3）基因修饰施万细胞(SCs)对神经干细胞体外分化为神经元样细胞的影响。方法：神经干细胞(NSCs)分别与NT-3基因修饰SCs（NT-3-SCs）、LacZ基因基因修饰SCs（LacZ-SCs）和SCs在体外共培养，7d后用免疫组化方法观测NSCs的分化并计算其中神经元样细胞的分化率。结果：NSCs在体外可分化为神经元样细胞（NF阳性）和神经胶质样细胞（GFAP阳性），与未基因修饰的SCs相比，NT-3-SCs能更有效地提高神经元样细胞的分化率，而LacZ-SCs与SCs没有明显区别。结论：NT-3-SCs能促进NSCs向神经元样细胞分化。
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| 关 键 词： | 干细胞 许旺细胞 神经营养素3 神经元样细胞 |
| 文章编号： | 1000-4718(2005)01-0026-05 |
| 收稿时间： | 2003-06-10 |
| 修稿时间： | 2003-09-15 |
NT-3 genetically modified Schwann cells promote neural stem cells to differentiate into neuron-like cells |
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| GUO Jia-song,ZENG Yuan-shan,LI Hai-biao,HUANG Wen-lin,LIU Ran-yi. NT-3 genetically modified Schwann cells promote neural stem cells to differentiate into neuron-like cells[J]. Chinese Journal of Pathophysiology, 2005, 21(1): 26-30. DOI: 1000-4718 |
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| Authors: | GUO Jia-song ZENG Yuan-shan LI Hai-biao HUANG Wen-lin LIU Ran-yi |
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| Affiliation: | 1Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College,2Cancer Center, Sun Yat-sen University, Guangzhou 510080, China;3Branch Campus of The First Military Medical University,Guangzhou 510315, China |
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| Abstract: | AIM: To explore the effects of neurotrophin-3 (NT-3)-genetically modified Schwann cells (NT-3-SCs) on differentiation of neural stem cells (NSCs) into the neuron-like cells. METHODS: The NSCs were co-cultured with NT-3-SCs. Report gene LacZ genetically modified Schwann cells (LacZ-SCs) and normal SCs respectively in vitro. 7 d later, the differentiation of NSCs was studied by immunohistochemistry, and the percentage of neuron-like cells was calculated. RESULTS: NSCs differentiated to the GFAP-positive cells (glial-like cells) and NF-positive cells (neuron-like cells) in vitro. Compared to the normal SCs, NT-3-SCs more efficiently promoted NSCs to differentiate into the neuron-like cells. The effect of LacZ-SCs was as the same to the normal SCs. CONCLUSION: NT-3-SCs promote NSCs to differentiate into the neuron-like cells. [ |
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| Keywords: | Stem cells Schwann cells Neurotrophin 3 Neuron-like cells |
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