癫痫持续状态患者神经元损伤与氧化应激的关系

目的 探讨癫痫持续状态(SE)患者神经元损伤与氧化应激的关系.方法 以30例SE患者(SE组)为研究对象,检测其在癫痫发作6 h内的血清神经元特异性烯醇化酶(NSE)、总超氧化物歧化酶(T-SOD)、8-羟基脱氧鸟苷酸(8-OHdG)及丙二醛(MDA)水平;以30例健康者作为对照组.结果 SE组血清NSE水平(26.98±4.16 ng/mL)高于对照组(16.34±3.11 ng/mL),T-SOD(77.22±18.42 U/mL)低于对照组(104.53±15.99 U/mL),8-OHdG(527.36±63.94 pg/mL)高于对照组(334.09±57.51 pg/mL),MDA(10.58±1.39 nmol/mL)高于对照组(5.33±1.76 nmol/mL)(P<0.001).SE组血清NSE水平与T-SOD呈负相关(r=-0.85,P<0.001),与8-OHdG、MDA呈正相关,相关系数分别为0.74和0.86(P<0.001).多元逐步回归分析显示,T-SOD、8-OHdG、MDA对NSE有显著影响,标准回归系数分别为-0.474、0.055、2.870(P<0.01).结论 SE患者体内存在氧化系统和抗氧化系统失衡,氧化应激可能参与了癫痫发作的病理生理过程,也是造成SE神经元损伤原因之一.

Relationship between injuries of neuron and oxidative stress in patients with status epilepticus

Objective To investigate the relationship between injuries of neuron and oxidative stress in patients with status epilepticus(SE).Methods Serum levels of neuron specific enolase(NSE),total superoxide dismutase(T-SOD),8-hydroxy-guanine(8-OHdG) and malondialdehyde(MDA)were detected in 30 cases of patients with SE(SE group) within 6 h after epileptic attack.30 cases of healthy subjects were enrolled as control group.Results Serum levels of NSE,8-OHdG and MDA in SE group were 26.98±4.16 ng/mL,527.36±63.94 pg/mL and 10.58±1.39 nmol/mL,which were respectively higher than 16.34±3.11 ng/mL,334.09±57.51 pg/mL and 5.33±1.76 nmol/mL in control group(P<0.001),but T-SOD level(77.22±18.42 U/mL) was lower than control group(104.53±15.99 U/mL)(P<0.001).In SE group,NSE was negatively correlated with T-SOD(r=-0.85,P<0.001),but positively correlated with 8-OHdG and MDA,correlation coefficients of which were 0.74 and 0.86,respectively(P<0.001).Serum levels of T-SOD,8-OHdG and MDA exerted significant effect on NSE,as demonstrated by multivariant stepwise regression analysis(P<0.01).Conclusion Oxidative stress might play a role in epileptic attack and might be an important risk factor in neuron injuries of SE.