不同原理尿液分析仪检测尿有形成分结果比较及显微镜复检规则探讨

目的 分析尿有形成分分析仪、尿干化学分析仪及显微镜尿沉渣结果 的差异,建立显微镜复检规则.方法 以UF-1000i尿有形成分分析仪及AX-4280尿干化学分析仪构成检测流水线,对临床尿标本先用流水线进行红细胞(RBC)、白细胞(WBC)及管型(CAST)检测,再用显微镜进行结果 验证.结果 UF-1000i检测RBC、WBC灵敏度高于AX-4280,但特异度低于AX-4280;流水线检测尿标本的阴性预测值达100.00%.UF-1000i分析仪与显微镜尿沉渣WBC、RBC、CAST检测结果 存在明显差异(χ2值分别为16.41、13.47、16.41,P＜0.05).建立适合该流水线的显微镜复检规则.结论 UF-1000i和AX-4280分析仪检测结果 有可能存在差异,此时不能以UF-1000i代替显微镜尿沉渣检查,需参照相应的复检规则进行复检,保证检测结果 准确性.

Comparison of the results of two different automatic urinalysis systems and construction of re-examination rules

Objective To analyze the difference between the results of UF-1000i urine sediment analyzer,AX-4280 urine dry chemistry analyzer and urine sediment test by microscopy,and to establish microscope re-examination rules.Methods RBC,WBC and CAST in urine samples were detected by UF-1000i and AX-4280,and then the results were confirmed by microscopy detection.Results The sensitivity of UF-1000i for the detection of RBC and WBC was higher than AX-4280,but specificity was lower.The negative predictive value of combined detection of the two analyzers was 100.00%.There was significant difference for the detection of WBC,RBC and CAST between UF-1000i and microscopy detection,with χ2 value of 16.41,13.47 and 16.41(P<0.05).The re-examination rules fit for this assembly line was established.Conclusion When there was difference between the results of UF-1000i and AX-4280,microscope detection should not be replaced completely by UF-1000i.Re-examination rules should be referred to perform re-examination to ensure the accuracy of test results.